| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UGT3A1 |
| Uniprot No | Q6NUS8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-523 aa |
| 活性数据 | MVGQRVLLLVAFLLSGVLLSEAAKILTISTLGGSHYLLLDRVSQILQEHGHNVTMLHQSGKFLIPDIKEEEKSYQVIRWFSPEDHQKRIKKHFDSYIETALDGRKESEALVKLMEIFGTQCSYLLSRKDIMDSLKNENYDLVFVEAFDFCSFLIAEKLVKPFVAILPTTFGSLDFGLPSPLSYVPVFPSLLTDHMDFWGRVKNFLMFFSFSRSQWDMQSTFDNTIKEHFPEGSRPVLSHLLLKAELWFVNSDFAFDFARPLLPNTVYIGGLMEKPIKPVPQDLDNFIANFGDAGFVLVAFGSMLNTHQSQEVLKKMHNAFAHLPQGVIWTCQSSHWPRDVHLATNVKIVDWLPQSDLLAHPSIRLFVTHGGQNSVMEAIRHGVPMVGLPVNGDQHGNMVRVVAKNYGVSIRLNQVTADTLTLTMKQVIEDKRYKSAVVAASVILHSQPLSPAQRLVGWIDHILQTGGATHLKPYAFQQPWHEQYLIDVFVFLLGLTLGTMWLCGKLLGVVARWLRGARKVKKT |
| 分子量 | 85.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UGT3A1蛋白的参考文献示例(注：文献信息经过简化整理，具体内容需根据实际检索补充)：
1. **文献名称**: "Identification and functional characterization of UDP-glycosyltransferase 3A1 as a novel steroid-conjugating enzyme"
**作者**: Meech, R., et al.
**摘要**: 该研究首次克隆并表达了重组人UGT3A1蛋白，发现其能够利用尿苷二磷酸葡萄糖(UDP-glucose)作为糖供体，催化雄激素和雌激素等类固醇的糖基化修饰，揭示了其在内源性代谢中的潜在作用。
2. **文献名称**: "Substrate specificity of human UDP-glucuronosyltransferase 3A1 (UGT3A1)"
**作者**: Sneitz, N., et al.
**摘要**: 通过体外重组表达UGT3A1.作者系统分析了其对多种药物和外源化合物的催化活性，发现其对酚类和黄酮类化合物具有选择性葡萄糖醛酸化能力，提示其在解毒途径中的独特功能。
3. **文献名称**: "The UDP-glucuronosyltransferase 3A family: divergent functions in human metabolism"
**作者**: Mackenzie, P.I., et al.
**摘要**: 本文综述了UGT3家族成员的进化与功能，重点提到UGT3A1的重组表达研究，表明其底物偏好性与传统UGT1/2家族不同，主要参与内源性物质而非药物的代谢。
4. **文献名称**: "Tissue distribution and enzymatic characterization of human UGT3A1"
**作者**: Takahashi, S., et al.
**摘要**: 研究利用重组UGT3A1蛋白进行酶动力学分析，并检测其在肝脏、肾脏和脑等组织的表达水平，结果显示其在神经类固醇代谢中可能发挥重要作用。
**注意**：以上文献为示例性内容，实际检索时建议通过PubMed或Google Scholar以“UGT3A1 recombinant protein”或“UGT3A1 functional characterization”为关键词查找最新研究，并确认作者与摘要的准确性。
Recombinant human UGT3A1 protein is a member of the uridine diphosphate (UDP)-glycosyltransferase (UGT) superfamily, which plays a critical role in phase II metabolism by catalyzing the transfer of glycosyl groups to endogenous and exogenous lipophilic molecules. UGT3A1. encoded by the UGT3A1 gene, is a lesser-studied isoform compared to the UGT1A and UGT2B subfamilies. It utilizes UDP-N-acetylglucosamine or UDP-glucose as sugar donors instead of the typical UDP-glucuronic acid, enabling unique substrate specificity for sterols, neurotransmitters, and other small molecules. This enzyme is expressed in various tissues, including the brain, liver, and kidneys, suggesting potential roles in neurosteroid regulation, drug metabolism, and detoxification pathways. Recombinant UGT3A1 is typically produced using heterologous expression systems (e.g., mammalian or insect cells) to maintain proper folding and enzymatic activity. Its study provides insights into atypical glycosylation mechanisms, interindividual variability in drug metabolism, and disease associations linked to dysregulated glycosylation, such as metabolic disorders or cancer. Current research focuses on characterizing its substrate spectrum, regulatory elements, and potential therapeutic applications in precision medicine.
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