| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TXNDC3 |
| Uniprot No | Q8N427 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-588 aa |
| 活性数据 | MASKKREVQL QTVINNQSLW DEMLQNKGLT VIDVYQAWCG PCRAMQPLFR KLKNELNEDE ILHFAVAEAD NIVTLQPFRD KCEPVFLFSV NGKIIEKIQG ANAPLVNKKV INLIDEERKI AAGEMARPQY PEIPLVDSDS EVSEESPCES VQELYSIAII KPDAVISKKV LEIKRKITKA GFIIEAEHKT VLTEEQVVNF YSRIADQCDF EEFVSFMTSG LSYILVVSQG SKHNPPSEET EPQTDTEPNE RSEDQPEVEA QVTPGMMKNK QDSLQEYLER QHLAQLCDIE EDAANVAKFM DAFFPDFKKM KSMKLEKTLA LLRPNLFHER KDDVLRIIKD EDFKILEQRQ VVLSEKEAQA LCKEYENEDY FNKLIENMTS GPSLALVLLR DNGLQYWKQL LGPRTVEEAI EYFPESLCAQ FAMDSLPVNQ LYGSDSLETA EREIQHFFPL QSTLGLIKPH ATSEQREQIL KIVKEAGFDL TQVKKMFLTP EQIEKIYPKV TGKDFYKDLL EMLSVGPSMV MILTKWNAVA EWRRLMGPTD PEEAKLLSPD SIRAQFGISK LKNIVHGASN AYEAKEVVNR LFEDPEEN |
| 分子量 | 67.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人TXNDC3蛋白的示例参考文献(内容为模拟概括，建议通过PubMed等数据库查询具体文献)：
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1. **文献名称**：*"TXNDC3: a novel thioredoxin-related protein involved in sperm maturation"*
**作者**：Miranda-Vizuete A. et al.
**摘要**：本研究报道TXNDC3在人类睾丸组织中的高表达，并通过体外实验证明其通过调控氧化还原状态影响精子鞭毛形成和运动功能，提示其在男性生殖系统中的关键作用。
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2. **文献名称**：*"TXNDC3 modulates ER stress and promotes cancer cell survival under hypoxia"*
**作者**：Park J.H. et al.
**摘要**：研究发现TXNDC3通过与内质网(ER)应激蛋白PDI互作，降低活性氧(ROS)水平，从而增强肿瘤细胞在缺氧条件下的存活能力，可能成为癌症治疗新靶点。
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3. **文献名称**：*"Structural characterization of the thioredoxin-like domain of TXNDC3 and its role in disulfide bond formation"*
**作者**：Andersson K.E. et al.
**摘要**：通过X射线晶体学解析TXNDC3蛋白结构，证实其硫氧还蛋白样结构域具有催化二硫键异构化活性，可能参与蛋白质折叠调控。
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**备注**：以上为示例性文献，实际文献请通过学术数据库(如PubMed、Web of Science)以“TXNDC3”或“thioredoxin domain-containing protein 3”为关键词检索。该蛋白的研究领域主要涉及生殖生物学、氧化应激及肿瘤耐药等。
The thioredoxin domain-containing protein 3 (TXNDC3), also known as sperm-specific thioredoxin-3. is a member of the thioredoxin superfamily characterized by a conserved thioredoxin-fold domain. It is primarily expressed in the testis, particularly during spermatogenesis, and plays a critical role in sperm maturation, motility, and structural integrity. TXNDC3 contains two distinct domains: an N-terminal thioredoxin domain with disulfide reductase activity, which participates in redox regulation, and a C-terminal domain resembling dynein light chain binding proteins, suggesting involvement in sperm flagellar assembly or function.
As a recombinant protein, recombinant human TXNDC3 (rhTXNDC3) is typically produced using prokaryotic or eukaryotic expression systems, enabling studies on its enzymatic properties and molecular interactions. It has been linked to male infertility, as mutations or aberrant expression of TXNDC3 are associated with impaired sperm motility (asthenozoospermia) and structural abnormalities. Beyond reproductive biology, emerging research implicates TXNDC3 in cancer progression and cellular stress responses, potentially acting through redox signaling pathways or cytoskeletal modulation. Its dual functional domains make it a unique target for investigating oxidative stress-related pathologies and developing diagnostic or therapeutic strategies for fertility disorders. Current studies focus on elucidating its mechanistic roles in spermiogenesis and disease contexts.
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