| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TWIST2 |
| Uniprot No | Q8WVJ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-160 aa |
| 活性数据 | MEEGSSSPVS PVDSLGTSEE ELERQPKRFG RKRRYSKKSS EDGSPTPGKR GKKGSPSAQS FEELQSQRIL ANVRERQRTQ SLNEAFAALR KIIPTLPSDK LSKIQTLKLA ARYIDFLYQV LQSDEMDNKM TSCSYVAHER LSYAFSVWRM EGAWSMSASH |
| 分子量 | 18.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TWIST2蛋白的3篇代表性文献的简要信息(注：部分文献内容为模拟概括，仅供参考)：
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1. **文献名称**: "Structural and functional characterization of human TWIST2 protein in epithelial-mesenchymal transition"
**作者**: Kim H, et al.
**摘要**: 研究解析了重组人TWIST2蛋白的晶体结构，揭示了其碱性螺旋-环-螺旋(bHLH)结构域的DNA结合机制，并证明其在调控上皮-间质转化(EMT)中的关键作用。
2. **文献名称**: "Overexpression of recombinant human TWIST2 promotes cancer cell invasion via MMP-9 activation"
**作者**: Zhang Y, et al.
**摘要**: 通过在大肠杆菌中重组表达TWIST2蛋白，发现其过表达可增强癌细胞侵袭能力，机制涉及对基质金属蛋白酶MMP-9的转录调控。
3. **文献名称**: "TWIST2 mutations associated with focal dermal hypoplasia disrupt Wnt/β-catenin signaling"
**作者**: Wang X, et al.
**摘要**: 研究利用重组突变型TWIST2蛋白，证明其与皮肤发育异常(如FCS综合征)相关突变会干扰Wnt/β-catenin通路，导致间充质干细胞分化异常。
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**注**：上述文献为示例，实际研究中建议通过PubMed或Web of Science以“TWIST2 recombinant protein”“TWIST2 function”为关键词检索最新论文。
**Background of Recombinant Human TWIST2 Protein**
TWIST2. a member of the basic helix-loop-helix (bHLH) transcription factor family, plays pivotal roles in embryonic development, cellular differentiation, and tissue homeostasis. It shares structural homology with TWIST1 but exhibits distinct expression patterns and regulatory functions. Primarily involved in mesenchymal cell lineage specification, TWIST2 modulates epithelial-mesenchymal transition (EMT), a process critical in organogenesis, wound healing, and cancer metastasis. By binding to E-box DNA sequences, TWIST2 regulates target genes associated with cell migration, proliferation, and apoptosis.
Dysregulation of TWIST2 is linked to pathological conditions. Overexpression is observed in certain cancers (e.g., soft tissue sarcomas, melanomas), promoting tumor progression and chemoresistance. Conversely, loss-of-function mutations cause autosomal recessive disorders like Setleis syndrome, characterized by craniofacial abnormalities. TWIST2 also influences immune responses, notably suppressing pro-inflammatory cytokine production in macrophages.
Recombinant TWIST2 protein, produced via bacterial or mammalian expression systems, retains DNA-binding activity and is used to study its molecular interactions, signaling pathways, and therapeutic potential. Its applications span mechanistic studies of EMT, cancer biology, and fibrosis, as well as drug screening for inhibitors targeting TWIST2-mediated oncogenesis.
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