| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TXNDC10 |
| Uniprot No | Q96JJ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-195 aa |
| 活性数据 | KGFVEDLDESFKENRNDDIWLVDFYAPWCGHCKKLEPIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIKLLKGDLAYNYRGPRTKDDIIEFAHRVSGALIRPLPSQQMFEHMQKRHRVFFVYVGGESPLKEKYIDAASELIVYTYFFSASEEVVPEVIFKI |
| 分子量 | 35.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TXNDC10蛋白的3篇代表性参考文献示例(内容为模拟虚构，仅供学术参考):
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### 1. **"TXNDC10 Promotes Hepatocellular Carcinoma Metastasis through Modulating ROS-mediated Apoptosis"**
**作者**: Li, X., et al.
**摘要**: 本研究揭示了重组人TXNDC10蛋白在肝癌细胞中通过调控活性氧(ROS)水平抑制细胞凋亡，进而促进肿瘤转移的机制。实验表明，过表达TXNDC10可增强癌细胞的侵袭能力，而敲低该蛋白则增加氧化应激诱导的凋亡。
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### 2. **"Structural and Functional Characterization of Recombinant Human TXNDC10 Protein"**
**作者**: Zhang, Y., & Wang, H.
**摘要**: 文章报道了通过大肠杆菌表达系统成功制备高纯度重组TXNDC10蛋白，并解析其晶体结构。功能实验显示，该蛋白具有硫氧还蛋白家族的典型抗氧化活性，且可通过二硫键异构酶活性参与内质网应激反应。
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### 3. **"TXNDC10 Regulates Autophagy via ER Stress Signaling Pathways in Colorectal Cancer"**
**作者**: Chen, J., et al.
**摘要**: 研究表明，重组TXNDC10蛋白在结直肠癌细胞中通过激活IRE1-XBP1信号通路，缓解内质网应激并抑制自噬，导致化疗耐药性增强。提示靶向TXNDC10可能成为提高治疗效果的新策略。
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(注：以上文献为假设性示例，实际研究请参考真实数据库如PubMed或Web of Science。)
**Background of Recombinant Human TXNDC10 Protein**
The thioredoxin domain-containing protein 10 (TXNDC10), also known as transmembrane thioredoxin-related protein (TMX3), is a member of the thioredoxin (Trx) superfamily characterized by a conserved Trx-fold structural motif. This protein is localized primarily in the endoplasmic reticulum (ER) and plays a role in redox homeostasis, protein folding, and quality control by facilitating disulfide bond formation or isomerization. TXNDC10 contains a single Trx-like domain with a CXXC active site motif, enabling its participation in thiol-disulfide exchange reactions, though its catalytic activity may differ from classical Trx proteins.
Studies suggest TXNDC10 is involved in cellular responses to ER stress, apoptosis regulation, and interactions with chaperones like protein disulfide isomerases (PDIs). It has been implicated in various pathological conditions, including cancer, neurodegenerative diseases, and metabolic disorders, where ER stress and oxidative damage are prominent. Recombinant human TXNDC10 protein, typically produced in *E. coli* or mammalian expression systems, is utilized to study its biochemical properties, substrate specificity, and mechanisms in redox signaling. Its application spans *in vitro* assays, structural studies, and therapeutic exploration targeting oxidative stress-related diseases. Further research is needed to fully elucidate its physiological roles and therapeutic potential.
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