| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBGCP4 |
| Uniprot No | Q9UGJ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-667 aa |
| 活性数据 | MIHELLLALS GYPGSIFTWN KRSGLQVSQD FPFLHPSETS VLNRLCRLGT DYIRFTEFIE QYTGHVQQQD HHPSQQGQGG LHGIYLRAFC TGLDSVLQPY RQALLDLEQE FLGDPHLSIS HVNYFLDQFQ LLFPSVMVVV EQIKSQKIHG CQILETVYKH SCGGLPPVRS ALEKILAVCH GVMYKQLSAW MLHGLLLDQH EEFFIKQGPS SGNVSAQPEE DEEDLGIGGL TGKQLRELQD LRLIEEENML APSLKQFSLR VEILPSYIPV RVAEKILFVG ESVQMFENQN VNLTRKGSIL KNQEDTFAAE LHRLKQQPLF SLVDFEQVVD RIRSTVAEHL WKLMVEESDL LGQLKIIKDF YLLGRGELFQ AFIDTAQHML KTPPTAVTEH DVNVAFQQSA HKVLLDDDNL LPLLHLTIEY HGKEHKADAT QAREGPSRET SPREAPASGW AALGLSYKVQ WPLHILFTPA VLEKYNVVFK YLLSVRRVQA ELQHCWALQM QRKHLKSNQT DAIKWRLRNH MAFLVDNLQY YLQVDVLESQ FSQLLHQINS TRDFESIRLA HDHFLSNLLA QSFILLKPVF HCLNEILDLC HSFCSLVSQN LGPLDERGAA QLSILVKGFS RQSSLLFKIL SSVRNHQINS DLAQLLLRLD YNKYYTQAGG TLGSFGM |
| 分子量 | 76.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TUBGCP4蛋白的3篇参考文献及其摘要内容：
1. **文献名称**：*Structural insights into γ-tubulin complex assembly using recombinant components*
**作者**：Zimmermann F., et al.
**摘要**：通过重组表达纯化的TUBGCP4与其他γ-TuRC复合体蛋白(如γ-tubulin、TUBGCP2/3)，解析了γ-TuRC在微管成核中的分子组装机制，揭示了TUBGCP4在复合体稳定性中的关键作用。
2. **文献名称**：*Functional characterization of TUBGCP4 mutations in microcephaly-related disorders*
**作者**：Martin C.A., et al.
**摘要**：研究重组人TUBGCP4蛋白在小头症相关突变中的功能影响，发现突变体导致γ-TuRC复合体功能障碍，从而破坏神经前体细胞的微管组织与分裂过程。
3. **文献名称**：*Reconstitution of the human γ-TuRC reveals distinct roles of TUBGCP4 in microtubule nucleation*
**作者**：Consolati T., et al.
**摘要**：利用重组TUBGCP4蛋白重构功能性γ-TuRC复合体，证明其作为调控亚基通过构象变化激活微管成核能力，并阐明其与癌症化疗药物结合的潜在位点。
---
注：上述文献为基于领域知识生成的示例，实际文献需通过数据库(如PubMed、Web of Science)检索验证。
**Background of Recombinant Human TUBGCP4 Protein**
TUBGCP4 (Tubulin Gamma Complex Component 4), also known as GCP4. is a critical subunit of the gamma-tubulin ring complex (γ-TuRC), a multi-protein assembly essential for microtubule nucleation and organization in eukaryotic cells. As part of γ-TuRC, TUBGCP4 facilitates the recruitment and stabilization of γ-tubulin, enabling the formation of microtubule networks that regulate cell division, intracellular transport, and maintenance of cell shape. Dysregulation of TUBGCP4 or γ-TuRC function has been linked to mitotic defects, genomic instability, and diseases such as cancer and neurodevelopmental disorders.
Recombinant human TUBGCP4 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and activity for research applications. Its recombinant form allows detailed study of γ-TuRC assembly, microtubule dynamics, and interactions with other regulatory proteins like CDK5RAP2 or MZT1. Researchers utilize this protein to explore mechanisms underlying microtubule-related pathologies, screen for small-molecule modulators, or analyze mutations affecting γ-TuRC function. Structural studies leveraging recombinant TUBGCP4 have also advanced understanding of its role in templating microtubule growth, offering insights into potential therapeutic strategies targeting microtubule-dependent processes.
×
