| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBG2 |
| Uniprot No | Q9NRH3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-451 aa |
| 活性数据 | MPREIITLQL GQCGNQIGFE FWKQLCAEHG ISPEGIVEEF ATEGTDRKDV FFYQADDEHY IPRAVLLDLE PRVIHSILNS PYAKLYNPEN IYLSEHGGGA GNNWASGFSQ GEKIHEDIFD IIDREADGSD SLEGFVLCHS IAGGTGSGLG SYLLERLNDR YPKKLVQTYS VFPYQDEMSD VVVQPYNSLL TLKRLTQNAD CVVVLDNTAL NRIATDRLHI QNPSFSQINQ LVSTIMSAST TTLRYPGYMN NDLIGLIASL IPTPRLHFLM TGYTPLTTDQ SVASVRKTTV LDVMRRLLQP KNVMVSTGRD RQTNHCYIAI LNIIQGEVDP TQVHKSLQRI RERKLANFIP WGPASIQVAL SRKSPYLPSA HRVSGLMMAN HTSISSLFES SCQQFDKLRK RDAFLEQFRK EDMFKDNFDE MDRSREVVQE LIDEYHAATQ PDYISWGTQE Q |
| 分子量 | 51.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TUBG2蛋白的假设参考文献示例(注：所列文献为虚构，仅供格式参考)：
1. **标题**: "Expression and Purification of Recombinant Human TUBG2 in E. coli for Structural Studies"
**作者**: Zhang, L. et al.
**摘要**: 本研究利用大肠杆菌表达系统成功表达并纯化了重组人TUBG2蛋白，通过X射线晶体学解析了其三维结构，揭示了其与微管组装相关的关键结构域。
2. **标题**: "TUBG2 Function in Mitotic Spindle Formation: Insights from Recombinant Protein Knockdown Assays"
**作者**: Müller, R. & Jensen, K.
**摘要**: 通过重组TUBG2蛋白的体外功能实验，结合siRNA敲低技术，证明TUBG2对有丝分裂中纺锤体的形成至关重要，其缺失导致染色体分离异常。
3. **标题**: "Role of TUBG2 in Glioblastoma: Recombinant Protein-Based Drug Screening"
**作者**: Chen, H. et al.
**摘要**: 基于重组TUBG2蛋白的高通量药物筛选，鉴定出两种小分子抑制剂可抑制胶质母细胞瘤细胞的异常增殖，为靶向治疗提供潜在策略。
4. **标题**: "Comparative Analysis of Post-Translational Modifications in Recombinant vs. Endogenous TUBG2"
**作者**: Santos, A. & Kim, T.
**摘要**: 利用质谱技术对比昆虫细胞表达的重组TUBG2与天然蛋白的翻译后修饰差异，揭示了重组表达系统对蛋白功能完整性的潜在影响。
**注意事项**：实际研究中请通过PubMed、Google Scholar等平台检索真实文献，关键词可尝试 "recombinant TUBG2" 或 "TUBG2 protein expression"。
Tubulin gamma-2 (TUBG2), a member of the γ-tubulin family, is a critical protein involved in microtubule nucleation and organization during cellular processes such as mitosis and meiosis. Unlike α- and β-tubulins that form microtubule polymers, γ-tubulin primarily functions within the γ-tubulin ring complex (γ-TuRC) to template microtubule assembly, ensuring proper spindle formation and chromosome segregation. TUBG2. encoded by the TUBG2 gene, shares high homology with its isoform TUBG1 but exhibits tissue-specific expression patterns, with higher levels observed in the brain, suggesting potential roles in neuronal development or maintenance.
Recombinant TUBG2 protein is engineered through heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) using DNA sequences optimized for high yield and stability. This allows researchers to study its biochemical properties, interactions with microtubule-associated proteins, and regulatory mechanisms in vitro. Purified recombinant TUBG2 is essential for structural studies (e.g., X-ray crystallography, cryo-EM) to map its binding domains and conformational changes during γ-TuRC assembly. Additionally, it serves as a tool to investigate dysregulation linked to diseases, including neurodevelopmental disorders and cancers, where aberrant microtubule dynamics contribute to pathogenesis. The protein’s recombinant form also aids in drug screening campaigns targeting mitotic errors or neurodegeneration. Recent studies emphasize its diagnostic and therapeutic potential, particularly in cancers with amplified TUBG2 expression.
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