| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBB4Q |
| Uniprot No | I0CMK4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-434 aa |
| 活性数据 | MRELVLTQTGQCGNQIGAKFWEVISDEHAIDSAGTYHGDSHLQLERINVHHHEASGGRYVSRAVLVDLEPGTMDSVRSGPFGQVFRPDNFISRQCGAGNNWAKGRYTEGAELTESVMDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIWEEYPDRIINTLSILLLPKVSDTVVEPYNATLSVHQLIENADETFCIDNEALYDICSRTLKLPTPTYGDLNHLVSATMSGVTTCLCFPDQLNADLRKLAMNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVAELTQQMFDAKNMMAARDPRHGRYLTAAAIFQGRMPMREVDEQMFNIQDKNSSYFADWFPNNVKTAVCDIPPWGLKMSVTFTGNNTAVQELKRVSEQFTATFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEGGGV |
| 分子量 | 74.69 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TUBB4Q蛋白的3篇参考文献概览：
1. **文献名称**：*TUBB4Q mutations cause adult-onset dystonia*
**作者**：Hersheson J, et al.
**摘要**：该研究发现TUBB4Q基因的特定突变与成人发病的肌张力障碍相关，首次揭示了该蛋白在神经系统微管功能中的关键作用，并通过体外重组蛋白实验表明突变影响微管稳定性。
2. **文献名称**：*Molecular characterization of TUBB4Q in cilia dysfunction disorders*
**作者**：Smith CM, et al.
**摘要**：本研究解析了TUBB4Q重组蛋白在纤毛形成中的作用，发现其表达异常导致纤毛结构缺陷，提示其与先天性纤毛病病理机制的相关性，并通过蛋白质互作分析揭示了其与其他微管蛋白的协同机制。
3. **文献名称**：*Recombinant TUBB4Q expression and in vitro polymerization assays*
**作者**：Lee H, Park J.
**摘要**：研究建立了重组人TUBB4Q蛋白的高效表达纯化系统，并证明该蛋白在体外能够自组装成微管结构，但其动力学特性与经典β-tubulin亚型存在差异，可能影响特定细胞类型的功能。
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**备注**：以上文献为示例性质，实际研究中TUBB4Q相关文献较少，建议结合关键词"TUBB4Q"与"microtubule"或相关疾病(如leukodystrophy)进一步检索PubMed等数据库以获取最新研究。
**Background of Recombinant Human TUBB4Q Protein**
TUBB4Q, a member of the β-tubulin family, is a class IV β-tubulin isotype encoded by the *TUBB4Q* gene in humans. Tubulins are critical structural components of microtubules, dynamic cytoskeletal polymers essential for cell division, intracellular transport, and maintenance of cell shape. β-tubulins, along with α-tubulins, form heterodimers that polymerize into microtubules, with their expression and post-translational modifications fine-tuning microtubule dynamics and functions.
TUBB4Q is notably tissue-specific, predominantly expressed in the testis and during spermatogenesis, suggesting a specialized role in male germ cell development. It is implicated in the structural and functional regulation of the sperm flagellum, which is vital for sperm motility. Dysregulation or mutations in TUBB4Q may contribute to impaired spermatogenesis or defects in sperm movement, potentially linking it to male infertility.
Compared to ubiquitously expressed β-tubulin isotypes (e.g., TUBB or TUBB4B), TUBB4Q exhibits unique sequence variations, possibly conferring distinct polymerization properties or interaction partners. Studies on recombinant TUBB4Q protein aid in dissecting its biochemical behavior, microtubule assembly dynamics, and role in specialized cellular processes. Its restricted expression also makes it a potential biomarker for germ cell-related pathologies or targets for fertility research. Further exploration of TUBB4Q could enhance understanding of tubulin isotype diversity and its implications in reproductive biology and disease.
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