| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBB4B |
| Uniprot No | P68371 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445 aa |
| 活性数据 | MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNNLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEEEAEEEVA |
| 分子量 | 74.47 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TUBB4B蛋白的三篇参考文献概述(注：文献为虚构示例，实际引用需查询真实数据库)：
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1. **文献名称**：*"Expression and Purification of Recombinant Human TUBB4B in Escherichia coli for Structural Studies"*
**作者**：Smith et al. (2019)
**摘要**：本研究报道了在大肠杆菌系统中高效表达重组人TUBB4B蛋白的优化方法，通过亲和层析和凝胶过滤纯化获得高纯度蛋白，并证实其可在体外组装成微管结构，为后续功能研究奠定基础。
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2. **文献名称**：*"TUBB4B Mutations Disrupt Microtubule Dynamics in Neurodevelopmental Disorders"*
**作者**：Zhang et al. (2020)
**摘要**：作者通过重组TUBB4B蛋白体外实验，结合荧光标记技术，揭示特定致病突变(如R391H)会削弱微管聚合能力，导致神经元轴突运输异常，阐释了TUBB4B突变引发脑发育障碍的分子机制。
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3. **文献名称**：*"Cryo-EM Structure of Recombinant TUBB4B Reveals Critical Regions for Tubulin Heterodimer Stability"*
**作者**：Johnson et al. (2021)
**摘要**：利用冷冻电镜解析重组人TUBB4B与α-微管蛋白形成的异源二聚体结构，鉴定了维持微管稳定性的关键氨基酸位点，为靶向微管相关疾病的药物设计提供结构依据。
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如需实际文献，建议在PubMed或Google Scholar中检索关键词“recombinant TUBB4B”、“TUBB4B protein expression”或结合疾病相关词(如“TUBB4B mutation”)。
The recombinant human TUBB4B protein is a engineered form of the β-tubulin isoform encoded by the *TUBB4B* gene, which belongs to the tubulin superfamily critical for cytoskeletal dynamics. As a core component of microtubules, β-tubulin heterodimerizes with α-tubulin to form protofilaments, enabling essential cellular processes like intracellular transport, cell division, and cilia formation. TUBB4B specifically regulates microtubule stability and organization in specialized tissues, notably influencing cochlear hair cell function and neuronal development.
Recombinant TUBB4B is typically produced in *E. coli* or mammalian expression systems, allowing high-purity isolation for structural and functional studies. Its production facilitates research into tubulin isotype-specific roles, particularly in neurodevelopmental disorders. Mutations in *TUBB4B* are linked to sensorineural hearing loss and ciliopathies, making the recombinant protein valuable for dissecting pathogenic mechanisms. Additionally, it serves as a tool for screening microtubule-targeting drugs or studying post-translational modifications like tyrosination/detyrosination that modulate microtubule interactions with motor proteins.
Studies using recombinant TUBB4B have clarified its distinct biochemical properties compared to other β-tubulin isoforms, such as GTP-binding affinity and polymerization kinetics, highlighting functional specialization within the tubulin family. Its availability accelerates research into microtubule-related diseases and therapeutic development.
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