| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBD1 |
| Uniprot No | Q9UJT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453 aa |
| 活性数据 | MSIVTVQLGQ CGNQIGFEVF DALLSDSHSS QGLCSMRENE AYQASCKERF FSEEENGVPI ARAVLVDMEP KVINQMLSKA AQSGQWKYGQ HACFCQKQGS GNNWAYGYSV HGPRHEESIM NIIRKEVEKC DSFSGFFIIM SMAGGTGSGL GAFVTQNLED QYSNSLKMNQ IIWPYGTGEV IVQNYNSILT LSHLYRSSDA LLLHENDAIH KICAKLMNIK QISFSDINQV LAHQLGSVFQ PTYSAESSFH YRRNPLGDLM EHLVPHPEFK MLSVRNIPHM SENSLAYTTF TWAGLLKHLR QMLISNAKME EGIDRHVWPP LSGLPPLSKM SLNKDLHFNT SIANLVILRG KDVQSADVEG FKDPALYTSW LKPVNAFNVW KTQRAFSKYE KSAVLVSNSQ FLVKPLDMIV GKAWNMFASK AYIHQYTKFG IEEEDFLDSF TSLEQVVASY CNL |
| 分子量 | 51.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TUBD1蛋白的参考文献示例(均为虚构，仅供格式参考)：
1. **"TUBD1 regulates centriole duplication through interaction with PLK4"**
*作者：Li, X. et al. (2020). Journal of Cell Biology.*
*摘要：* 本研究通过重组TUBD1蛋白的体外实验，证实其与激酶PLK4的相互作用，并揭示其在中心体复制中的调控机制，为细胞周期异常相关疾病提供潜在靶点。
2. **"Expression and purification of recombinant human TUBD1 for structural analysis"**
*作者：Smith, J. & Patel, R. (2018). Protein Expression and Purification.*
*摘要：* 报道了一种高效表达和纯化重组人TUBD1蛋白的方案，利用大肠杆菌系统获得高纯度蛋白，并验证其适用于X射线晶体学研究。
3. **"TUBD1 mutations disrupt microtubule dynamics in ciliopathies"**
*作者：Garcia, M. et al. (2021). Nature Communications.*
*摘要：* 通过重组TUBD1突变体功能实验，发现其突变导致微管动力学异常，与纤毛功能障碍相关疾病(如多囊肾)密切相关。
4. **"Cryo-EM structure of the TUBD1-containing γ-tubulin ring complex"**
*作者：Chen, Z. et al. (2019). Cell Structure.*
*摘要：* 利用冷冻电镜解析包含重组TUBD1的γ-微管蛋白环复合体(γ-TuRC)结构，阐明其在微管成核过程中的分子机制。
(注：以上文献为示例性虚构内容，实际研究中请查询具体数据库获取真实文献。)
**Background of Recombinant Human TUBD1 Protein**
Tubulin Delta Complex 1 (TUBD1) is a member of the tubulin superfamily, specifically categorized as a δ-tubulin isoform. It plays a critical role in microtubule organization and mitotic spindle assembly, contributing to cell division and structural integrity during mitosis. Unlike canonical α- and β-tubulins, δ-tubulin is a less-studied variant implicated in centriole and basal body formation, which are essential for cilia/flagella assembly and proper cell cycle progression.
Recombinant human TUBD1 protein is engineered using heterologous expression systems, such as *E. coli* or insect cells*,* to produce a purified, biologically active form. This allows researchers to study its molecular interactions, structural properties, and functional mechanisms in vitro. TUBD1 interacts with other tubulin isoforms and microtubule-associated proteins (MAPs) to stabilize microtubule networks and regulate their dynamics. Dysregulation of TUBD1 has been linked to developmental disorders, ciliopathies, and potential oncogenic processes due to its role in ensuring accurate chromosome segregation and genetic stability.
Studies leveraging recombinant TUBD1 have advanced our understanding of its contributions to centrosome duplication, mitotic fidelity, and ciliary function. Its recombinant form is also utilized in high-throughput drug screening to identify therapeutic agents targeting microtubule-related pathologies, including cancer and congenital anomalies. Ongoing research continues to unravel TUBD1's broader regulatory roles in cellular physiology and disease.
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