| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBGCP3 |
| Uniprot No | Q96CW5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-907 aa |
| 活性数据 | MATPDQKSPNVLLQNLCCRILGRSEADVAQQFQYAVRVIGSNFAPTVERDEFLVAEKIKKELIRQRREADAALFSELHRKLHSQGVLKNKWSILYLLLSLSEDPRRQPSKVSSYATLFAQALPRDAHSTPYYYARPQTLPLSYQDRSAQSAQSSGSVGSSGISSIGLCALSGPAPAPQSLLPGQSNQAPGVGDCLRQQLGSRLAWTLTANQPSSQATTSKGVPSAVSRNMTRSRREGDTGGTMEITEAALVRDILYVFQGIDGKNIKMNNTENCYKVEGKANLSRSLRDTAVRLSELGWLHNKIRRYTDQRSLDRSFGLVGQSFCAALHQELREYYRLLSVLHSQLQLEDDQGVNLGLESSLTLRRLLVWTYDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMRSLVQHILSLVSHPVLSFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYTLRKSMIPSFMTMDQSRKVLLIGKSINFLHQVCHDQTPTTKMIAVTKSAESPQDAADLFTDLENAFQGKIDAAYFETSKYLLDVLNKKYSLLDHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQHNLTGILETAVRATNAQFDSPEILRRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTRECMSHYLRVFNFLWRAKRMEYILTDIRKGHMCNAKLLRNMPEFSGVLHQCHILASEMVHFIHQMQYYITFEVLECSWDELWNKVQQAQDLDHIIAAHEVFLDTIISRCLLDSDSRALLNQLRAVFDQIIELQNAQDAIYRAALEELQRRLQFEEKKKQREIEGQWGVTAAEEEEENKRIGEFKESIPKMCSQLRILTHFYQGIVQQFLVLLTTSSDESLRFLSFRLDFNEHYKAREPRLRVSLGTRGRRSSHT |
| 分子量 | 130 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Assembly of recombinant human gamma-tubulin complex proteins"**
- Authors: Murphy, S.M. et al.
- 摘要：研究利用重组表达的TUBGCP3与其他γ-微管蛋白复合体组分(如GCP2/GCP4)进行体外组装实验，揭示其在微管成核中的核心作用及复合体稳定性机制。
2. **"Structural insights into the γ-tubulin complex: Role of GCP3 in microtubule nucleation"**
- Authors: Teixidó-Travesa, N. et al.
- 摘要：通过冷冻电镜解析γ-TuRC复合体中TUBGCP3的结构，阐明其与γ-微管蛋白的相互作用界面及对微管生长方向调控的功能。
3. **"CRISPR-based functional analysis identifies TUBGCP3 as a critical regulator of mitotic spindle integrity"**
- Authors: Zheng, Y. et al.
- 摘要：采用CRISPR敲除技术证明TUBGCP3缺失导致纺锤体异常和细胞周期停滞，并通过回补重组TUBGCP3蛋白恢复表型，确认其在有丝分裂中的必要性。
4. **"Quantitative proteomics of GCP proteins in human cell lines"**
- Authors: Hutchins, J.R. et al.
- 摘要：基于质谱的定量分析揭示TUBGCP3在癌细胞中表达上调，并与化疗耐药性相关，提示其作为潜在治疗靶点的价值。
(注：以上文献为示例，实际引用需根据具体论文调整。建议通过PubMed或Web of Science以“TUBGCP3”和“recombinant”为关键词检索最新研究。)
TUBGCP3 (Tubulin Gamma Complex Component 3), also known as GCP3. is a critical subunit of the gamma-tubulin ring complex (γ-TuRC), a multi-protein assembly essential for microtubule nucleation in eukaryotic cells. This complex serves as a template for initiating microtubule growth, playing a pivotal role in organizing the mitotic spindle during cell division. TUBGCP3 interacts with other GCP family proteins (GCP2. GCP4-6) and gamma-tubulin to form a structural scaffold that stabilizes microtubule minus-ends, ensuring proper spindle orientation and chromosomal segregation. Dysregulation of TUBGCP3 has been linked to mitotic errors, genomic instability, and pathologies including cancer and neurodevelopmental disorders. Studies suggest its involvement in centrosome maturation and cell cycle progression, mediated through phosphorylation signaling pathways. Recombinant human TUBGCP3 protein, typically produced in bacterial or eukaryotic expression systems, is widely used to investigate γ-TuRC assembly mechanisms, dissect microtubule dynamics, and screen compounds targeting mitotic machinery. Its structural and functional conservation across species makes it a valuable model for evolutionary studies of cytoskeletal regulation.
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