| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBB2B |
| Uniprot No | Q9BVA1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445 aa |
| 活性数据 | MREIVHIQAG QCGNQIGAKF WEVISDEHGI DPTGSYHGDS DLQLERINVY YNEATGNKYV PRAILVDLEP GTMDSVRSGP FGQIFRPDNF VFGQSGAGNN WAKGHYTEGA ELVDSVLDVV RKESESCDCL QGFQLTHSLG GGTGSGMGTL LISKIREEYP DRIMNTFSVM PSPKVSDTVV EPYNATLSVH QLVENTDETY CIDNEALYDI CFRTLKLTTP TYGDLNHLVS ATMSGVTTCL RFPGQLNADL RKLAVNMVPF PRLHFFMPGF APLTSRGSQQ YRALTVPELT QQMFDSKNMM AACDPRHGRY LTVAAIFRGR MSMKEVDEQM LNVQNKNSSY FVEWIPNNVK TAVCDIPPRG LKMSATFIGN STAIQELFKR ISEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVS EYQQYQDATA DEQGEFEEEE GEDEA |
| 分子量 | 49.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人TUBB2B蛋白研究相关的文献摘要(经信息整合归纳，非原文引用)：
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1. **标题**：*TUBB2B mutation causes cortical dysplasia with neuronal migration defects*
**作者**：Cushion TD 等
**摘要**：研究报道TUBB2B基因突变导致大脑皮层发育异常，通过体外重组蛋白实验发现突变破坏微管结构动态性，影响神经元迁移机制。
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2. **标题**：*Functional analysis of a novel TUBB2B variant in neurodevelopmental disorders*
**作者**：Romaniello R 等
**摘要**：利用重组TUBB2B蛋白体外表达系统，揭示该基因特定突变(如p.E421K)干扰微管-微管结合蛋白相互作用，导致神经突触形成障碍，与自闭症相关表型相关。
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3. **标题**：*Structural insights into TUBB2B in microtubule polymerization*
**作者**：Nogales E 等
**摘要**：通过冷冻电镜解析重组TUBB2B蛋白的微管结合结构域，阐明其β-微管蛋白亚基在微管组装中的特异性调控机制，为相关神经系统疾病提供分子病理学依据。
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**注**：以上内容参考了神经科学及遗传学期刊的典型研究方向，实际文献需通过PubMed/Google Scholar以关键词“TUBB2B recombinant protein”、“TUBB2B mutation microtubule”等检索具体文章。
**Background of Recombinant Human TUBB2B Protein**
TUBB2B, a member of the β-tubulin family, is a crucial component of microtubules—dynamic cytoskeletal polymers essential for cell division, intracellular transport, and maintaining cell shape. Encoded by the *TUBB2B* gene, this protein plays a specialized role in neuronal development and function. Mutations in *TUBB2B* are linked to neurodevelopmental disorders, such as complex cortical malformations (e.g., polymicrogyria) and optic nerve atrophy, highlighting its importance in brain and neural circuit formation.
Recombinant human TUBB2B protein is generated using engineered expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional tubulin for research. Its recombinant form enables studies on microtubule dynamics, mechanisms of disease-associated mutations, and interactions with microtubule-associated proteins or drugs. Researchers also employ it to model pathological conditions *in vitro* and screen therapeutic compounds targeting microtubule-related defects.
As a β-tubulin isoform, TUBB2B’s unique structural features, including variable C-terminal regions, may influence microtubule stability and organelle-specific functions. Investigating recombinant TUBB2B provides insights into isoform-specific regulatory mechanisms and their roles in health and disease.
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