| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTLL10 |
| Uniprot No | Q6ZVT0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-673 aa |
| 活性数据 | MDHSCTRFIH RRGPPTRTRA GFKRGKRPRI QQRPRARVSG TIPASRLHPA PASQPGPCPA PGHCPVGPAH ERPMGSSQEE GLRCQPSQPD HDADGHCGPD LEGAERASAT PGPPGLLNSH RPADSDDTNA AGPSAALLEG LLLGGGKPSP HSTRPGPFFY IGGSNGATII SSYCKSKGWQ RIHDSRRDDY TLKWCEVKSR DSYGSFREGE QLLYQLPNNK LLTTKIGLLS TLRGRARAMS KASKVPGGVQ ARLEKDAAAP ALEDLPWTSP GYLRPQRVLR MEEFFPETYR LDLKHEREAF FTLFDETQIW ICKPTASNQG KGIFLLRNQE EVAALQAKTR SMEDDPIHHK TPFRGPQARV VQRYIQNPLL VDGRKFDVRS YLLIACTTPY MIFFGHGYAR LTLSLYDPHS SDLGGHLTNQ FMQKKSPLYM LLKEHTVWSM EHLNRYISDT FWKARGLAKD WVFTTLKKRM QQIMAHCFLA AKPKLDCKLG YFDLIGCDFL IDDNFKVWLL EMNSNPALHT NCEVLKEVIP GVVIETLDLV LETFRKSLRG QKMLPLLSQR RFVLLHNGEA DPRPHLGGSC SLRRWPPLPT RQAKSSGPPM PHAPDQPGAR RPAPPPLVPQ RPRPPGPDLD SAHDGEPQAP GTEQSGTGNR HPAQEPSPGT AKEEREEPEN ARP |
| 分子量 | 75.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTLL10蛋白的3篇参考文献的示例(注：文献标题和内容基于公开研究背景模拟，具体文献需通过数据库核实)：
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1. **"Biochemical characterization of human TTLL10 as a tubulin polyglutamylase"**
*作者：Ikegami K, Setou M*
**摘要**：该研究首次报道了TTLL10作为微管蛋白聚谷氨酸化酶的功能，利用重组人TTLL10蛋白验证其体外催化微管C端谷氨酸链延长的活性，并解析了其底物特异性。
2. **"Recombinant expression and functional analysis of TTLL10 in neuronal differentiation"**
*作者：Li Y, Janke C, et al.*
**摘要**：通过在HEK293细胞中表达重组TTLL10.研究发现其通过修饰微管动力学调控神经元轴突生长，提示其在神经发育中的潜在作用。
3. **"Structural insights into the catalytic mechanism of TTLL10 through cryo-EM"**
*作者：Zhang R, Nogales E*
**摘要**：利用冷冻电镜解析重组TTLL10蛋白的三维结构，揭示其底物结合域和催化核心的分子机制，为设计靶向抑制剂提供基础。
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**建议**：
- 实际文献请通过PubMed、Google Scholar等平台检索关键词“TTLL10 recombinant”或结合研究方向(如“tubulin modification”)筛选。
- 如无法获取全文，可使用学术工具(如ResearchGate)联系作者或使用机构访问权限。
TTLL10 (Tubulin Tyrosine Ligase-Like family member 10) is a member of the TTLL enzyme family, which is involved in post-translational modifications of tubulins, particularly glutamylation and tyrosination. These modifications regulate microtubule dynamics, stability, and interactions with motor proteins, influencing critical cellular processes such as cell division, intracellular transport, and cilia formation. TTLL10 specifically functions as a polyglutamylase, catalyzing the addition of glutamate chains to the γ-carboxyl group of specific tubulin residues. This activity contributes to the structural diversity of microtubules, enabling tissue-specific functional specialization.
Studies have linked TTLL10 to cilia-related functions, with implications in developmental biology and human diseases. For instance, it plays roles in spermatogenesis by affecting flagellar structure in sperm and is associated with ciliopathies when dysregulated. Recombinant human TTLL10 protein is commonly produced in heterologous expression systems (e.g., E. coli or mammalian cells) for biochemical and functional studies. Its structural characterization helps elucidate substrate specificity and catalytic mechanisms within the TTLL family. Current research focuses on its regulatory roles in cellular physiology, potential as a therapeutic target in cilia dysfunction disorders, and interactions with microtubule-associated proteins. However, its tissue-specific expression patterns and precise regulatory pathways remain areas of active investigation.
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