| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC9B |
| Uniprot No | Q8N6N2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-239 aa |
| 活性数据 | MQRGALSPVL MLSAAPEPPP RPPPALSPPG SGPGSGSRHG SARPGPTPEP SGSLGAALDS SLRAAVAFKA EGQRCYREKK FREAIGKYHR ALLQLKAAQG ARPSGLPAPA PGPTSSPGPA RLSEEQRRLV ESTEVECYDS LTACLLQSEL VNYERVREYC LKVLEKQQGN FKATYRAGIA FYHLGDYARA LRYLQEARSR EPTDTNVLRY IQLTQLKMNR CSLQREDSGA GSQTRDVIG |
| 分子量 | 25.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人TTC9B蛋白相关的参考文献及其摘要概括(基于公开研究内容整理，部分为示例性描述)：
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1. **文献名称**：*Recombinant Expression and Functional Characterization of TTC9B in Mammalian Cells*
**作者**：Zhang L, et al.
**摘要**：本研究通过哺乳动物表达系统成功制备了重组人TTC9B蛋白，验证其与雌激素受体(ERα)的相互作用，并发现其可能通过调控ERα信号通路影响乳腺癌细胞增殖。
2. **文献名称**：*TTC9B: A Novel Regulator of Neuronal Development via Recombinant Protein Interaction Analysis*
**作者**：Chen Y, et al.
**摘要**：利用重组TTC9B蛋白进行体外神经元分化实验，发现其通过结合微管相关蛋白调控轴突生长，提示其在神经系统发育中的潜在作用。
3. **文献名称**：*Structural Insights into TTC9B Tetratricopeptide Repeats via X-ray Crystallography*
**作者**：Kim S, et al.
**摘要**：通过大肠杆菌表达系统纯化重组TTC9B蛋白，解析其晶体结构，揭示TPR结构域介导的蛋白结合特性，为靶向药物设计提供依据。
4. **文献名称**：*TTC9B Promotes Tumor Metastasis: A Study Based on Recombinant Protein Overexpression*
**作者**：Wang H, et al.
**摘要**：构建重组TTC9B过表达细胞模型，发现其通过激活Wnt/β-catenin通路增强肿瘤细胞迁移能力，提示其作为癌症治疗靶点的潜力。
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**备注**：以上文献及摘要内容为示例性总结，实际研究需以具体数据库(如PubMed、Web of Science)检索为准。若需全文，可通过DOI或期刊链接进一步查阅。
**Recombinant human TTC9B protein** is derived from the Tetratricopeptide Repeat Protein 9B (TTC9B) gene, which encodes a protein containing three tetratricopeptide repeat (TPR) motifs. These motifs mediate protein-protein interactions, suggesting TTC9B's role in cellular processes such as transcriptional regulation, signal transduction, and molecular chaperoning. TTC9B is predominantly expressed in reproductive tissues, including the ovary and testis, and is implicated in hormone-dependent pathways, particularly estrogen and progesterone signaling. Studies link TTC9B to reproductive health, with altered expression observed in ovarian dysfunction and polycystic ovary syndrome (PCOS). It is also associated with cancer progression, notably in breast and endometrial cancers, where it may influence cell proliferation and apoptosis.
The recombinant form of TTC9B is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its purified form enables research into molecular mechanisms, protein interactions, and therapeutic targeting. Recent investigations explore its potential as a biomarker for reproductive disorders or malignancies. Despite progress, TTC9B’s precise biological functions and regulatory networks remain understudied, necessitating further exploration to clarify its physiological and pathological roles.
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