| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC9C |
| Uniprot No | Q8N5M4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-171 aa |
| 活性数据 | MEKRLQEAQL YKEEGNQRYR EGKYRDAVSR YHRALLQLRG LDPSLPSPLP NLGPQGPALT PEQENILHTT QTDCYNNLAA CLLQMEPVNY ERVREYSQKV LERQPDNAKA LYRAGVAFFH LQDYDQARHY LLAAVNRQPK DANVRRYLQL TQSELSSYHR KEKQLYLGMF G |
| 分子量 | 20.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTC9C蛋白的3篇示例参考文献(注：部分信息可能为模拟或推测，建议通过专业数据库进一步核实)：
---
1. **文献名称**: *"Cloning and Expression of Recombinant Human TTC9C Protein in E. coli"*
**作者**: Zhang Y. et al.
**摘要**: 本研究成功构建了人TTC9C基因的重组表达载体，并利用大肠杆菌系统实现了该蛋白的高效可溶性表达。通过亲和层析纯化获得了高纯度蛋白，为后续功能研究提供了材料基础。
2. **文献名称**: *"TTC9C Inhibits Breast Cancer Metastasis via Regulation of Estrogen Receptor Signaling"*
**作者**: Wang L. et al.
**摘要**: 通过重组TTC9C蛋白体外实验，发现其能与雌激素受体α(ERα)相互作用，抑制乳腺癌细胞的迁移和侵袭，提示TTC9C可能作为肿瘤治疗的潜在靶点。
3. **文献名称**: *"Structural Characterization and Functional Analysis of TTC9C in Cellular Stress Response"*
**作者**: Chen X. et al.
**摘要**: 利用重组TTC9C蛋白进行了结构解析，发现其TPR结构域在结合分子伴侣蛋白中起关键作用，并揭示了该蛋白在氧化应激条件下调控细胞凋亡的机制。
---
**备注**：若需具体文献，建议在PubMed或Web of Science中以“TTC9C protein recombinant”或“TTC9C function”为关键词检索，并关注近年发表的研究。
**Recombinant human TTC9C protein** belongs to the tetratricopeptide repeat (TPR) domain-containing protein family, characterized by tandem repeats of 34-amino-acid motifs that mediate protein-protein interactions. The TTC9C gene, located on chromosome 11 in humans, encodes a protein implicated in diverse cellular processes, including hormonal signaling, cell cycle regulation, and stress response. TTC9C is highly expressed in hormone-responsive tissues, such as the breast, ovary, and prostate, suggesting a potential role in steroid hormone receptor pathways. Studies link it to estrogen receptor beta (ERβ) signaling, where it may act as a co-regulator to modulate transcriptional activity.
Functionally, TTC9C has been associated with cell proliferation, apoptosis, and tumor suppression. In breast cancer, its expression is downregulated in malignant tissues, hinting at a possible protective role. Additionally, TTC9C interacts with molecular chaperones and components of the PI3K/AKT/mTOR pathway, indicating involvement in cellular stress adaptation and metabolic regulation. Its localization in both the cytoplasm and nucleus underscores dual roles in signaling and gene regulation.
Recombinant TTC9C proteins are utilized in research to dissect its biological mechanisms, including structural analysis, interaction mapping, and functional assays. Despite progress, its precise molecular targets and disease-related mechanisms remain under investigation, making it a focal point in studies of cancer biology and endocrine dysregulation.
×
