| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC5 |
| Uniprot No | Q8N0Z6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-440 aa |
| 活性数据 | MMADEEEEVK PILQKLQELV DQLYSFRDCY FETHSVEDAG RKQQDVQKEM EKTLQQMEEV VGSVQGKAQV LMLTGKALNV TPDYSPKAEE LLSKAVKLEP ELVEAWNQLG EVYWKKGDVA AAHTCFSGAL THCRNKVSLQ NLSMVLRQLR TDTEDEHSHH VMDSVRQAKL AVQMDVHDGR SWYILGNSYL SLYFSTGQNP KISQQALSAY AQAEKVDRKA SSNPDLHLNR ATLHKYEESY GEALEGFSRA AALDPAWPEP RQREQQLLEF LDRLTSLLES KGKVKTKKLQ SMLGSLRPAH LGPCSDGHYQ SASGQKVTLE LKPLSTLQPG VNSGAVILGK VVFSLTTEEK VPFTFGLVDS DGPCYAVMVY NIVQSWGVLI GDSVAIPEPN LRLHRIQHKG KDYSFSSVRV ETPLLLVVNG KPQGSSSQAV ATVASRPQCE |
| 分子量 | 48.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTC5蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: **"TTC5 interacts with p53 and regulates its function in stress response"**
**作者**: Kawai, H., et al.
**摘要**: 本研究揭示了TTC5通过与肿瘤抑制蛋白p53直接结合，调控其在DNA损伤应激下的转录活性。研究者利用重组人TTC5蛋白进行体外互作实验，证明TTC5对p53介导的细胞周期停滞和凋亡具有调控作用。
2. **文献名称**: **"Structural insights into the TTC5 co-chaperone protein and its interaction with HSP70"**
**作者**: Smith, J.R., et al.
**摘要**: 通过X射线晶体学解析了重组人TTC5蛋白的三维结构，发现其TPR结构域介导与HSP70分子伴侣的相互作用。实验表明TTC5可能作为辅伴侣蛋白参与应激条件下的蛋白折叠调控。
3. **文献名称**: **"Recombinant expression and functional characterization of human TTC5 in cell proliferation"**
**作者**: Zhang, Y., et al.
**摘要**: 该研究在大肠杆菌中成功表达并纯化重组人TTC5蛋白，功能实验显示其过表达抑制癌细胞增殖，并通过调节细胞周期相关基因(如Cyclin D1)发挥作用。
(注：以上文献为示例性质，实际引用时需根据具体研究核实信息。)
TTC5 (Tetratricopeptide Repeat Domain 5) is a human protein characterized by its namesake tetratricopeptide repeat (TPR) motifs, which are structural elements that mediate protein-protein interactions. Belonging to the TPR-containing protein family, TTC5 is evolutionarily conserved and ubiquitously expressed across tissues, suggesting fundamental roles in cellular processes. It has been implicated in transcriptional regulation, signal transduction, and cell cycle control, often acting as a cofactor or adaptor molecule. Structurally, its TPR domains enable scaffolding functions, facilitating the assembly of multiprotein complexes.
Research highlights TTC5’s interaction with key regulators, including the tumor suppressor p53. where it may modulate p53-dependent transcriptional activity under stress conditions. Dysregulation of TTC5 has been linked to cancer progression, such as prostate and liver cancer, potentially through pathways affecting apoptosis and proliferation. Additionally, TTC5 is associated with neurodevelopmental disorders, with studies suggesting its involvement in neural differentiation and synaptic function. Mutations in the TTC5 gene have been correlated with intellectual disabilities and autism spectrum disorders.
Recombinant human TTC5 protein, produced via expression systems like *E. coli* or mammalian cells, serves as a critical tool for elucidating its biochemical properties, interactome, and therapeutic potential. Its study continues to uncover novel mechanisms in cellular homeostasis and disease pathogenesis.
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