| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC6 |
| Uniprot No | Q86TZ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520 aa |
| 活性数据 | MMKYYDLAKF TIYQIAEMDK GLSELSPMQQ ALIYSFCENH DKAIEVLDGI SWNRAEMTMC ALLAKVQMKA KRTKEAVEVL KKALDAISHS DKGPDATAIS ADCLYNLGLC YMEEGNLQMT YKLAITDLTT AISMDKNSYT AFYNRALCYT KIRELQMALT DYGIVLLLDA TETVKLNTFL NRGLIYVELG QYGFALEDFK QAALISRTNG SLCHATAMCH HRINEFEEAV NFFTWALKIN PCFLDAYVGR GNSYMEYGHD EATKQAQKDF LKALHINPAY IKARISFGYN LQAQGKFQKA WNHFTIAIDT DPKNYLAYEG RAVVCLQMGN NFAAMQDINA AMKISTTAEF LTNRGVIHEF MGHKQNAMKD YQDAITLNPK YSLAYFNAGN IYFHHRQFSQ ASDYFSKALK FDPENEYVLM NRAITNTILK KYEEAKEDFA NVIESCPFWA AVYFNRAHFY YCLKQYELAE EDLNKALSLK PNDALVYNFR AKVRGKIGLI EEAMADYNQA LDLEDYASVI |
| 分子量 | 59.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTC6蛋白的参考文献示例(基于领域常见研究方向，部分内容为假设性概括，若需真实文献请通过学术数据库检索)：
1. **《Cloning, Expression, and Purification of Recombinant Human TTC6 in Escherichia coli》**
**作者**: Smith A et al.
**摘要**: 本研究描述了通过大肠杆菌系统克隆和表达重组人TTC6蛋白，优化诱导条件以提高可溶性表达，并利用His标签亲和层析进行纯化，为后续功能研究提供高纯度蛋白。
2. **《Structural Insights into TTC6-HSP90 Interaction by X-ray Crystallography》**
**作者**: Lee C et al.
**摘要**: 通过X射线晶体学解析了TTC6与HSP90伴侣蛋白复合物的结构，揭示了其TPR结构域介导的相互作用机制，阐明了TTC6在HSP90功能调控中的关键作用。
3. **《TTC6 Modulates Apoptosis via Regulation of Bcl-2 Family Proteins》**
**作者**: Garcia R et al.
**摘要**: 发现重组TTC6蛋白通过与Bcl-2家族成员互作，影响线粒体途径的细胞凋亡，研究采用体外结合实验和基因敲除模型验证了其促凋亡功能。
4. **《Functional Characterization of TTC6 in Cell Cycle Progression》**
**作者**: Tanaka M et al.
**摘要**: 利用重组TTC6蛋白进行功能研究，发现其过表达导致G1/S期阻滞，机制涉及p53信号通路激活，提示TTC6在细胞周期调控中的潜在作用。
**备注**：以上文献为示例性质，真实研究需通过PubMed、Google Scholar等平台检索关键词“recombinant TTC6”或“human TTC6 protein”获取最新进展。若需具体文献，建议查阅相关数据库。
The tetratricopeptide repeat protein 6 (TTC6) is a member of the TPR protein family, characterized by tandem repeats of 34 amino acid motifs that mediate protein-protein interactions. TTC6 contains multiple TPR domains, enabling it to act as a scaffold or adaptor in cellular processes such as signal transduction, chaperone activity, and protein trafficking. It is ubiquitously expressed in human tissues and implicated in regulating the cell cycle, apoptosis, and stress responses. Studies suggest TTC6 interacts with heat shock proteins (e.g., HSP90), influencing client protein folding and stability. Its role in modulating pathways like Wnt/β-catenin and NF-κB highlights potential links to cancers, neurodegenerative diseases, and developmental disorders.
Recombinant human TTC6 protein is produced using expression systems like *E. coli* or mammalian cells, allowing precise study of its structure and function. This engineered protein retains binding capabilities crucial for investigating molecular mechanisms and identifying interaction partners. Research applications include exploring TTC6's regulatory roles in diseases, screening therapeutic targets, and developing diagnostic tools. Dysregulation of TTC6 has been observed in conditions such as intellectual disability and liver cancer, underscoring its biomedical relevance. By providing a purified, bioactive form, recombinant TTC6 facilitates biochemical assays, structural studies, and antibody production, advancing understanding of its physiological and pathological contributions.
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