| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC4 |
| Uniprot No | O95801 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-387 aa |
| 活性数据 | MEQPGQDPTS DDVMDSFLEK FQSQPYRGGF HEDQWEKEFE KVPLFMSRAP SEIDPRENPD LACLQSIIFD EERSPEEQAK TYKDEGNDYF KEKDYKKAVI SYTEGLKKKC ADPDLNAVLY TNRAAAQYYL GNFRSALNDV TAARKLKPCH LKAIIRGALC HLELKHFAEA VNWCDEGLQI DAKEKKLLEM RAKADKLKRI EQRDVRKANL KEKKERNQNE ALLQAIKARN IRLSEAACED EDSASEGLGE LFLDGLSTEN PHGARLSLDG QGRLSWPVLF LYPEYAQSDF ISAFHEDSRF IDHLMVMFGE TPSWDLEQKY CPDNLEVYFE DEDRAELYRV PAKSTLLQVL QHQRYFVKAL TPAFLVCVGS SPFCKNFLRG RKVYQIR |
| 分子量 | 44.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTC4蛋白的示例性参考文献，基于研究领域的合理推断整理而成。实际文献需通过学术数据库(如PubMed、Google Scholar)进一步验证：
---
1. **标题**：*"Expression and Purification of Recombinant Human TTC4 in Escherichia coli for Functional Studies"*
**作者**：Li, X., et al.
**摘要**：该研究成功构建了人源TTC4的原核表达系统，通过优化诱导条件和亲和层析技术获得高纯度蛋白，验证其适用于后续相互作用及结构分析。
2. **标题**：*"TTC4 as a Novel Co-chaperone of HSP90: Mechanistic Insights into Protein Folding"*
**作者**：García-Ranea, J.A., et al.
**摘要**：通过重组TTC4蛋白的体外结合实验，发现其与HSP90和FKBP52形成复合物，提示其在信号转导和蛋白折叠中作为辅助伴侣的作用。
3. **标题**：*"TTC4 Modulates ERK Signaling Pathway in Breast Cancer Cells via Recombinant Protein Delivery"*
**作者**：Wang, Y., et al.
**摘要**：使用重组TTC4蛋白处理乳腺癌细胞，发现其通过调控ERK通路抑制肿瘤增殖，为靶向治疗提供潜在策略。
4. **标题**：*"Structural Characterization of Human TTC4 by X-ray Crystallography"*
**作者**：Zhang, L., et al.
**摘要**：基于重组TTC4蛋白的晶体结构解析，揭示了其四肽重复(TPR)结构域的关键功能位点，为突变研究奠定基础。
---
**建议**：具体文献可通过关键词 **"recombinant human TTC4"** 或 **"TTC4 protein function"** 在学术平台检索，重点关注蛋白表达、伴侣功能及疾病关联研究。
**Background of Recombinant Human TTC4 Protein**
The Tetratricopeptide repeat protein 4 (TTC4), a member of the TPR protein family, is characterized by tandem TPR motifs—structural domains that mediate protein-protein interactions and complex assembly. TTC4 is ubiquitously expressed in human tissues and plays roles in diverse cellular processes, including chaperone-assisted protein folding, cell cycle regulation, and stress response. It interacts with heat shock proteins (e.g., HSP70/90) and other chaperones, suggesting involvement in maintaining proteostasis.
Recombinant human TTC4 protein is produced using biotechnological systems (e.g., *E. coli* or mammalian cells*) to enable functional studies. Its production allows exploration of structural-functional relationships, particularly how TPR motifs coordinate partner proteins. Research links TTC4 to cancer, as altered expression is observed in breast and ovarian cancers, potentially influencing tumor progression via pathways like p53 signaling. Additionally, TTC4 variants are associated with neurodevelopmental disorders, underscoring its importance in cellular health.
Studying recombinant TTC4 provides insights into its role in disease mechanisms and therapeutic targeting, such as modulating chaperone networks or restoring dysregulated pathways in malignancies. Further investigation is needed to fully define its interactome and pathophysiological relevance.
×
