| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSSC1 |
| Uniprot No | Q53HC9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-387 aa |
| 活性数据 | MEDDAPVIYGLEFQARALTPQTAETDAIRFLVGTQSLKYDNQIHIIDFDDENNIINKNVLLHQAGEIWHISASPADRGVLTTCYNRTSDSKVLTCAAVWRMPKELESGSHESPDDSSSTAQTLELLCHLDNTAHGNMACVVWEPMGDGKKIISLADNHILLWDLQESSSQAVLASSASLEGKGQLKFTSGRWSPHHNCTQVATANDTTLRGWDTRSMSQIYCIENAHGQLVRDLDFNPNKQYYLASCGDDCKVKFWDTRNVTEPVKTLEEHSHWVWNVRYNHSHDQLVLTGSSDSRVILSNMVSISSEPFGHLVDDDDISDQEDHRSEEKSKEPLQDNVIATYEEHEDSVYAVDWSSADPWLFASLSYDGRLVINRVPRALKYHILL |
| 分子量 | 70 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TSSC1蛋白的3条虚构参考文献，格式包含文献名称、作者及摘要概括：
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1. **文献名称**: *"Recombinant Human TSSC1 Protein Induces Apoptosis in Osteosarcoma Cells via p53 Activation"*
**作者**: Chen L, Wang Y, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人TSSC1蛋白，通过体外实验证明其能够通过激活p53通路诱导骨肉瘤细胞凋亡，为TSSC1的肿瘤抑制功能提供了直接证据。
2. **文献名称**: *"Structural and Functional Characterization of TSSC1 in the Regulation of mTOR Signaling"*
**作者**: Kumar R, Gupta S.
**摘要**: 通过X射线晶体学解析重组TSSC1蛋白的结构，揭示其与mTOR复合物的相互作用界面，并证明TSSC1过表达通过抑制mTOR信号通路延缓肿瘤生长。
3. **文献名称**: *"TSSC1 Recombinant Protein Interacts with DNA-PKcs to Enhance Genomic Stability"*
**作者**: Martinez D, Lee J.
**摘要**: 利用重组TSSC1蛋白进行Pull-down实验，发现其与DNA-PKcs直接结合，增强DNA损伤修复能力，提示其在维持基因组稳定性中的关键作用。
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以上内容为示例性质，实际文献需通过数据库(如PubMed/Google Scholar)检索确认。
**Background of Recombinant Human TSSC1 Protein**
TSSC1 (Tumor Suppressing Subtransferable Candidate 1), also known as *TRIM77*, is a protein encoded by a gene located in the imprinted 11p15.5 chromosomal region, which is linked to growth regulation and tumor suppression. This region is associated with Beckwith-Wiedemann syndrome (BWS), a congenital disorder characterized by overgrowth and cancer predisposition. TSSC1 belongs to the TRIM (Tripartite Motif) protein family, which typically contains RING, B-box, and coiled-coil domains, though TSSC1 lacks the canonical RING domain. It is hypothesized to act as a tumor suppressor by regulating cell proliferation, apoptosis, or genomic stability, potentially through interactions with imprinted genes like *IGF2* or pathways such as the IGF signaling axis.
Recombinant human TSSC1 protein is produced using biotechnological systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant form allows researchers to explore its structure, biochemical properties, and role in development and diseases like cancer. Studies suggest that TSSC1 may counteract oncogenic processes, though its precise mechanisms remain under investigation. Challenges in studying TSSC1 include its low endogenous expression and complex regulation within imprinted gene clusters. Current research focuses on elucidating its interactions with neighboring tumor suppressors (e.g., TSSC4) and oncogenes, as well as its potential as a therapeutic target. Recombinant TSSC1 tools are critical for advancing understanding of 11p15.5-related disorders and developing diagnostic or treatment strategies.
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