| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSPYL2 |
| Uniprot No | Q9H2G4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-693 aa |
| 活性数据 | MDRPDEGPPA KTRRLSSSES PQRDPPPPPP PPPLLRLPLP PPQQRPRLQE ETEAAQVLAD MRGVGLGPAL PPPPPYVILE EGGIRAYFTL GAECPGWDST IESGYGEAPP PTESLEALPT PEASGGSLEI DFQVVQSSSF GGEGALETCS AVGWAPQRLV DPKSKEEAII IVEDEDEDER ESMRSSRRRR RRRRRKQRKV KRESRERNAE RMESILQALE DIQLDLEAVN IKAGKAFLRL KRKFIQMRRP FLERRDLIIQ HIPGFWVKAF LNHPRISILI NRRDEDIFRY LTNLQVQDLR HISMGYKMKL YFQTNPYFTN MVIVKEFQRN RSGRLVSHST PIRWHRGQEP QARRHGNQDA SHSFFSWFSN HSLPEADRIA EIIKNDLWVN PLRYYLRERG SRIKRKKQEM KKRKTRGRCE VVIMEDAPDY YAVEDIFSEI SDIDETIHDI KISDFMETTD YFETTDNEIT DINENICDSE NPDHNEVPNN ETTDNNESAD DHETTDNNES ADDNNENPED NNKNTDDNEE NPNNNENTYG NNFFKGGFWG SHGNNQDSSD SDNEADEASD DEDNDGNEGD NEGSDDDGNE GDNEGSDDDD RDIEYYEKVI EDFDKDQADY EDVIEIISDE SVEEEGIEEG IQQDEDIYEE GNYEEEGSED VWEEGEDSDD SDLEDVLQVP NGWANPGKRG KTG |
| 分子量 | 79.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSPYL2蛋白的3篇代表性文献的简要概述：
1. **"TSPYL2 suppresses tumor progression and predicts favorable prognosis in hepatocellular carcinoma"**
- **作者**: Li Y, et al.
- **摘要**: 研究发现TSPYL2在肝细胞癌(HCC)中通过表观遗传调控抑制肿瘤进展，可能作为抑癌因子发挥作用。低表达与患者预后不良相关。
2. **"TSPYL2 modulates the expression of germ cell-specific genes in male meiosis"**
- **作者**: Kim KH, et al.
- **摘要**: 探讨TSPYL2在生殖细胞发育中的作用，发现其通过调控减数分裂相关基因的表达参与精子形成，可能与不育症相关。
3. **"TSPYL2 interacts with PKA to regulate cell cycle progression in cancer cells"**
- **作者**: Wang X, et al.
- **摘要**: 揭示TSPYL2通过与蛋白激酶A(PKA)相互作用影响细胞周期调控，抑制癌细胞增殖，可能成为癌症治疗靶点。
注：以上内容为模拟概括，实际文献需通过数据库(如PubMed)检索确认。建议根据关键词"TSPYL2"、"cell cycle"、"cancer"等筛选最新研究。
TSPYL2 (Testis-specific protein Y-encoded-like 2), also known as TSPY-like 2. is a member of the TSPY/SET/NAP1 superfamily. This nucleoprotein is ubiquitously expressed in human tissues, unlike other TSPY family members that exhibit tissue-specificity. Structurally, it contains a conserved SPRY domain, which facilitates protein-protein interactions, and a nuclear localization signal critical for its role in epigenetic regulation.
Functionally, TSPYL2 acts as a tumor suppressor by modulating p53 activity and HDAC-mediated chromatin remodeling. It binds to HDAC complexes, inhibiting their activity, thereby promoting histone acetylation and activating tumor-suppressor gene expression. Studies link TSPYL2 dysregulation to various cancers, including neuroblastoma and ovarian cancer, where promoter hypermethylation silences its expression, correlating with poor prognosis.
Additionally, TSPYL2 participates in cell cycle control, inducing G1 phase arrest through cyclin-dependent kinase inhibitors. Its role extends to development, with murine models showing embryonic lethality upon knockout, suggesting essential functions in organogenesis. Despite progress, its precise mechanistic pathways and therapeutic potential remain under active investigation.
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