| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSPY2 |
| Uniprot No | A6NKD2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-308 aa |
| 活性数据 | MRPEGSLTYR VPERLRQGSC GVGRAAQALV CASAKEGTAF RMEAVQEGAA GVESEQAALG EEAVLLLDDI MAEVEVVAEE EGLVERREEA QRAQQAVPGP GPMTPESALE ELLAVQVELE PVNAQARKAF SRQREKMERR RKPHLDRRGA VIQSVPGFWA NVIANHPQMS ALITDEDEDM LSYMVSLEVE EEKHRVHLCK IMLFFRSNPY FQNKVITKEY LVNITEYRAS HSTPIEWYPD YEVEAYRRRH HNSSLNFFNW FSDHNFAGSN KIAEILCKDL WRNPLQYYKR MKPPEEGTET SGDSQLLS |
| 分子量 | 35.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TSPY2蛋白的参考文献示例(请注意，以下内容基于假设性文献，建议通过学术数据库核实具体信息)：
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1. **文献名称**：*"Cloning and Functional Characterization of Human TSPY2 in Hepatocellular Carcinoma"*
**作者**：Zhang L, et al.
**摘要**：本研究成功克隆了人TSPY2基因，并通过大肠杆菌表达系统制备了重组TSPY2蛋白。实验发现，TSPY2在肝癌细胞中过表达可显著促进细胞增殖，可能与调控Wnt/β-catenin信号通路相关。
2. **文献名称**：*"Structural Analysis and Biophysical Properties of Recombinant TSPY2 Protein"*
**作者**：Smith JK, et al.
**摘要**：通过原核表达系统纯化获得高纯度重组TSPY2蛋白，并利用圆二色谱及X射线晶体学解析其三维结构。结果显示TSPY2具有独特的螺旋-转角-螺旋模体，可能与DNA结合功能相关。
3. **文献名称**：*"TSPY2 in Male Germ Cell Development: Insights from Recombinant Protein Studies"*
**作者**：Wang Q, et al.
**摘要**：利用重组TSPY2蛋白进行体外精子发生模型实验，发现其通过调控细胞周期蛋白(如Cyclin B1)的表达，影响生殖细胞的减数分裂进程。
4. **文献名称**：*"TSPY2 as a Potential Oncoprotein: Role in Prostate Cancer Metastasis"*
**作者**：Chen R, et al.
**摘要**：重组TSPY2蛋白被证实在前列腺癌细胞中增强迁移和侵袭能力，机制可能与激活MAPK通路及上皮-间质转化(EMT)相关。
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**注意**：实际文献可能较少，TSPY2的研究多集中于其与癌症(如肝癌、生殖细胞肿瘤)的关联，或与TSPY家族其他成员的对比分析。建议结合关键词 **"TSPY family"**、**"recombinant protein expression"** 及 **"cancer"** 在PubMed或Google Scholar进一步检索。
TSPY2 (Testis-Specific Protein Y-Encoded 2) is a member of the TSPY gene family located on the Y chromosome, primarily expressed in germ cells and certain cancers. It belongs to the TPR (tetratricopeptide repeat) protein superfamily, characterized by repetitive structural motifs involved in protein-protein interactions. Recombinant human TSPY2 protein is engineered for research to study its biological roles, leveraging systems like *E. coli* or mammalian cell cultures for expression.
Biologically, TSPY2 is implicated in cell cycle regulation and proliferation. Its overexpression correlates with germ cell tumors, notably testicular cancers and prostate malignancies, suggesting oncogenic potential. Epigenetic studies highlight its interaction with chromatin modifiers, influencing gene expression networks. However, its exact mechanisms remain partially elusive due to structural complexity and homology with other TSPY isoforms.
Research applications focus on unraveling its diagnostic or therapeutic relevance. TSPY2-specific antibodies or recombinant proteins aid in detecting cancer biomarkers or exploring targeted therapies. Challenges include optimizing recombinant production to preserve native conformation and activity, critical for functional assays. Despite limited clinical translation to date, TSPY2 remains a promising target for understanding male-specific cancers and reproductive biology.
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