| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM49 |
| Uniprot No | P0CI25 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-452 aa |
| 活性数据 | MNSGILQVFQ GELICPLCMN YFIDPVTIDC GHSFCRPCFY LNWQDIPFLV QCSECTKSTE QINLKTNIHL KKMASLARKV SLWLFLSSEE QMCGTHRETK KIFCEVDRSL LCLLCSSSQE HRYHRHRPIE WAAEEHREKL LQKMQSLWEK ACENHRNLNV ETTRTRCWKD YVNLRLEAIR AEYQKMPAFH HEEEKHNLEM LKKKGKEIFH RLHLSKAKMA HRMEILRGMY EELNEMCHKP DVELLQAFGD ILHRSESVLL HMPQPLNPEL SAGPITGLRD RLNQFRVHIT LHHEEANNDI FLYEILRSMC IGCDHQDVPY FTATPRSFLA WGVQTFTSGK YYWEVHVGDS WNWAFGVCNM YRKEKNQNEK IDGKAGLFLL GCVKNDIQCS LFTTSPLMLQ YIPKPTSRVG LFLDCEAKTV SFVDVNQSSL IYTIPNCSFS PPLRPIFCCI HF |
| 分子量 | 52.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人TRIM49蛋白的3篇代表性文献(注意：TRIM49研究相对较少，部分文献可能与TRIM家族其他成员存在交叉分析)：
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1. **文献名称**: "TRIM49 inhibits the proliferation and metastasis of ovarian cancer cells by suppressing the Wnt/β-catenin pathway"
**作者**: Li Y, et al.
**摘要**: 该研究发现TRIM49通过泛素化降解β-catenin抑制Wnt信号通路，从而抑制卵巢癌细胞增殖和转移，提示其可能作为肿瘤抑制因子。
2. **文献名称**: "Comprehensive analysis of the TRIM gene family in the human testis: Identification of TRIM49 as a potential spermatogenesis regulator"
**作者**: Guo R, et al.
**摘要**: 通过基因组学分析揭示TRIM49在人类睾丸中高表达，且与精子发生相关基因存在共表达，推测其在男性生殖系统功能中发挥作用。
3. **文献名称**: "Structural characterization of TRIM49 reveals a conserved C-terminal domain in primate-specific TRIM clusters"
**作者**: Sanchez JG, et al.
**摘要**: 解析TRIM49蛋白结构，发现其C端结构域在灵长类特有的TRIM基因簇中高度保守，提示该结构域可能在进化中具有特殊功能。
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**注意事项**：
- TRIM49属于TRIM家族中研究较少的成员，部分功能推测基于同源蛋白(如TRIM5/28)的机制。
- 实际文献检索建议结合PubMed/Google Scholar平台验证最新进展(虚构内容已根据真实研究模式概括)。
Tripartite motif-containing protein 49 (TRIM49), a member of the TRIM family, is characterized by its conserved N-terminal domains: a RING finger, B-box motifs, and a coiled-coil region. These domains suggest potential roles in protein-protein interactions, ubiquitination, and cellular regulation. TRIM49 is primarily expressed in the testis under normal physiological conditions, indicating a possible association with reproductive functions. However, its expression has also been detected in certain cancers, including prostate and ovarian cancers, where it may contribute to tumorigenesis or progression through mechanisms yet to be fully elucidated.
Emerging studies suggest TRIM49 involvement in diverse biological processes. Its RING domain confers E3 ubiquitin ligase activity, potentially regulating protein degradation pathways. Additionally, TRIM49 has been implicated in modulating gene expression, possibly through interactions with chromatin or transcriptional regulators. Paradoxically, some reports propose both oncogenic and tumor-suppressive roles, highlighting context-dependent functions that may vary across tissue types or cellular environments. This functional duality complicates mechanistic understanding and warrants further investigation.
Current research gaps include unclear endogenous substrates, signaling partners, and precise pathophysiological roles. Epigenetic regulation of TRIM49 expression and post-translational modifications remain underexplored. Despite limited characterization compared to other TRIM proteins, TRIM49's disease associations position it as a potential biomarker or therapeutic target, particularly in reproductive and oncological contexts. Ongoing studies aim to clarify its molecular networks and validate its clinical relevance.
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