| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM47 |
| Uniprot No | Q96LD4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-638 aa |
| 活性数据 | MDGSGPFSCP ICLEPLREPV TLPCGHNFCL ACLGALWPHR GASGAGGPGG AARCPLCQEP FPDGLQLRKN HTLSELLQLR QGSGPGSGPG PAPALAPEPS APSALPSVPE PSAPCAPEPW PAGEEPVRCD ACPEGAALPA ALSCLSCLAS FCPAHLGPHE RSPALRGHRL VPPLRRLEES LCPRHLRPLE RYCRAERVCL CEACAAQEHR GHELVPLEQE RALQEAEQSK VLSAVEDRMD ELGAGIAQSR RTVALIKSAA VAERERVSRL FADAAAALQG FQTQVLGFIE EGEAAMLGRS QGDLRRQEEQ RSRLSRARQN LSQVPEADSV SFLQELLALR LALEDGCGPG PGPPRELSFT KSSQAVRAVR DMLAVACVNQ WEQLRGPGGN EDGPQKLDSE ADAEPQDLES TNLLESEAPR DYFLKFAYIV DLDSDTADKF LQLFGTKGVK RVLCPINYPL SPTRFTHCEQ VLGEGALDRG TYYWEVEIIE GWVSMGVMAE DFSPQEPYDR GRLGRNAHSC CLQWNGRSFS VWFHGLEAPL PHPFSPTVGV CLEYADRALA FYAVRDGKMS LLRRLKASRP RRGGIPASPI DPFQSRLDSH FAGLFTHRLK PAFFLESVDA HLQIGPLKKS CISVLKRR |
| 分子量 | 69.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM47蛋白的模拟参考文献示例(实际文献需通过学术数据库查询)：
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1. **文献名称**: *TRIM47 promotes tumor progression via ubiquitination of PTEN in colorectal cancer*
**作者**: Zhang Y, et al.
**摘要**: 本研究揭示TRIM47在结直肠癌中通过泛素化修饰PTEN蛋白，抑制其稳定性，激活AKT/mTOR通路，促进肿瘤增殖和转移，提示其作为潜在治疗靶点。
2. **文献名称**: *Structural basis of TRIM47 ubiquitin ligase activity in antiviral immunity*
**作者**: Li H, et al.
**摘要**: 通过冷冻电镜解析TRIM47蛋白结构，阐明其RING结构域介导的泛素化机制，并发现其通过干扰病毒衣壳蛋白降解增强宿主先天免疫反应。
3. **文献名称**: *TRIM47 as a prognostic biomarker in hepatocellular carcinoma*
**作者**: Wang X, et al.
**摘要**: 分析肝癌患者样本发现TRIM47高表达与不良预后相关，其表达水平可独立预测患者生存率，机制涉及调控细胞周期蛋白Cyclin D1.
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建议通过 **PubMed** 或 **Web of Science** 检索最新文献，关键词：TRIM47 AND (cancer/ubiquitination/signaling) 。
TRIM47 (Tripartite Motif-Containing Protein 47) is a member of the TRIM protein family, characterized by a conserved N-terminal tripartite motif comprising a RING finger domain, one or two B-box domains, and a coiled-coil region. These domains enable TRIM proteins to function as E3 ubiquitin ligases, regulating processes like protein degradation, immune response, and cell proliferation. TRIM47. located on human chromosome 17q25.3. is expressed in various tissues, with elevated levels observed in certain cancers, neurodegenerative disorders, and cardiovascular diseases. Its biological roles remain incompletely defined, but studies suggest involvement in tumor progression, apoptosis regulation, and inflammatory pathways.
Research highlights TRIM47's dual role in cancer: it may act as an oncogene or tumor suppressor depending on cellular context. For example, TRIM47 overexpression promotes proliferation, metastasis, and chemoresistance in colorectal and lung cancers by activating Wnt/β-catenin signaling or stabilizing oncoproteins via ubiquitination. Conversely, its downregulation in glioblastoma correlates with poor prognosis. Beyond oncology, TRIM47 interacts with viral proteins, modulating host antiviral responses, and has been implicated in neurological conditions like Parkinson’s disease through protein aggregation pathways. Despite growing interest, mechanistic insights into its physiological functions and disease-specific regulation remain limited. Current efforts focus on exploring TRIM47 as a diagnostic biomarker or therapeutic target, though further validation in model systems is required.
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