| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM49L2 |
| Uniprot No | P0CI26 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-452 aa |
| 活性数据 | MNSGILQVFQ GELICPLCMN YFIDPVTIDC GHSFCRPCFY LNWQDIPFLV QCSECTKSTE QINLKTNIHL KKMASLARKV SLWLFLSSEE QMCGTHRETK KIFCEVDRSL LCLLCSSSQE HRYHRHRPIE WAAEEHREKL LQKMQSLWEK ACENHRNLNV ETTRTRCWKD YVNLRLEAIR AEYQKMPAFH HEEEKHNLEM LKKKGKEIFH RLHLSKAKMA HRMEILRGMY EELNEMCHKP DVELLQAFGD ILHRSESVLL HMPQPLNPEL SAGPITGLRD RLNQFRVHIT LHHEEANSDI FLYEILRSMC IGCDHQDVPY FTATPRSFLA WGVQTFTSGK YYWEVHVGDS WNWAFGVCNM YRKEKNQNEK IDGKEGLFLL GCIKNDIQCS LFTTSPLMLQ YIPKPTSRVG LFLDCEAKTV SFVDVNQSSL IYTIPNCSFS PPLRPIFCCI HF |
| 分子量 | 52.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM49L2蛋白的**虚构参考文献示例**(仅供参考，实际文献需通过数据库检索确认)：
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1. **标题**: *TRIM49L2 regulates spermatogenesis through ubiquitination of histone variants*
**作者**: Kasagi Y, et al. (2018)
**摘要**: 研究发现TRIM49L2在小鼠睾丸中高表达，通过泛素化修饰组蛋白变体参与染色质重塑，影响精子形成，敲除后导致雄性不育。
2. **标题**: *Structural insights into TRIM49L2's role in antiviral response*
**作者**: Zhang R, et al. (2019)
**摘要**: 该研究解析了TRIM49L2的晶体结构，发现其通过RING结构域介导泛素化，抑制HIV-1病毒衣壳蛋白的组装，提出其作为先天免疫调节因子的功能。
3. **标题**: *TRIM49L2 promotes lung adenocarcinoma progression via PI3K/AKT activation*
**作者**: Wang X, et al. (2020)
**摘要**: 在肺癌细胞中，TRIM49L2通过泛素化降解PTEN蛋白，激活PI3K/AKT信号通路，促进肿瘤增殖和转移，提示其作为潜在治疗靶点。
4. **标题**: *Evolutionary analysis of TRIM49L2 in primates*
**作者**: Baranova E, et al. (2021)
**摘要**: 通过对灵长类基因组的比较，发现TRIM49L2在人类中经历正向选择，可能与宿主防御或生殖系统适应性进化相关。
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**注意**：以上内容为示例性虚构，实际研究中TRIM49L2的功能可能有所不同。建议通过PubMed、Google Scholar等平台检索真实文献。
TRIM49L2 (Tripartite Motif-Containing Protein 49-Like 2) is a member of the TRIM protein family, characterized by conserved RING, B-box, and coiled-coil domains. This ubiquitously expressed protein is primarily localized in the cytoplasm and nucleus, playing roles in diverse cellular processes such as apoptosis, transcriptional regulation, and innate immunity. While its exact biological functions remain under investigation, TRIM49L2 is hypothesized to participate in protein ubiquitination, a key post-translational modification regulating protein stability, interactions, and signaling. Emerging studies link TRIM49L2 to cancer progression and viral defense mechanisms, though mechanistic insights are limited.
The recombinant human TRIM49L2 protein, typically produced in bacterial or mammalian expression systems, enables in vitro studies of its structural and functional properties. Researchers utilize it to explore substrate interactions, enzymatic activities (e.g., E3 ubiquitin ligase potential), and regulatory pathways. Structural analyses reveal variations in its C-terminal regions compared to other TRIM49 subfamily members, suggesting functional diversification. Its gene, located on chromosome 19q13.43. shows evolutionary conservation and tissue-specific expression patterns. Current research focuses on clarifying its physiological targets, disease associations, and therapeutic potential in autoimmune disorders or infections. Recombinant TRIM49L2 serves as a critical tool for dissecting its molecular role in cellular homeostasis and pathology.
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