| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM45 |
| Uniprot No | Q9H8W5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-580 aa |
| 活性数据 | MSENRKPLLG FVSKLTSGTA LGNSGKTHCP LCLGLFKAPR LLPCLHTVCT TCLEQLEPFS VVDIRGGDSD TSSEGSIFQE LKPRSLQSQI GILCPVCDAQ VDLPMGGVKA LTIDHLAVND VMLESLRGEG QGLVCDLCND REVEKRCQTC KANLCHFCCQ AHRRQKKTTY HTMVDLKDLK GYSRIGKPIL CPVHPAEELR LFCEFCDRPV CQDCVVGEHR EHPCDFTSNV IHKHGDSVWE LLKGTQPHVE ALEEALAQIH IINSALQKRV EAVAADVRTF SEGYIKAIEE HRDKLLKQLE DIRAQKENSL QLQKAQLEQL LADMRTGVEF TEHLLTSGSD LEILITKRVV VERLRKLNKV QYSTRPGVND KIRFCPQEKA GQCRGYEIYG TINTKEVDPA KCVLQGEDLH RAREKQTASF TLLCKDAAGE IMGRGGDNVQ VAVVPKDKKD SPVRTMVQDN KDGTYYISYT PKEPGVYTVW VCIKEQHVQG SPFTVMVRRK HRPHSGVFHC CTFCSSGGQK TARCACGGTM PGGYLGCGHG HKGHPGHPHW SCCGKFNEKS ECTWTGGQSA PRSLLRTVAL |
| 分子量 | 64.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TRIM45蛋白的3篇参考文献摘要示例：
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1. **文献名称**：*TRIM45 regulates neuronal apoptosis via ubiquitination of Bim*
**作者**：Yamauchi T et al.
**摘要**：该研究通过在大肠杆菌中表达重组人TRIM45蛋白，证实其作为E3泛素连接酶的功能，能够在神经元中促进促凋亡蛋白Bim的泛素化及降解，调节神经细胞的生存与凋亡。
2. **文献名称**：*Structural insights into TRIM45-mediated antiviral signaling*
**作者**：Li X et al.
**摘要**：通过纯化重组TRIM45蛋白并结合X射线晶体学分析，揭示了其N端RING结构域与泛素结合的关键位点，阐明了TRIM45在抗病毒天然免疫通路中激活干扰素表达的分子机制。
3. **文献名称**：*TRIM45 interacts with protein kinase C and modulates NF-κB activity*
**作者**：Miyamoto Y et al.
**摘要**：利用HEK293细胞表达重组TRIM45蛋白，发现其通过泛素化修饰PKCα并抑制NF-κB信号通路，提示其在炎症反应中的负调控作用。
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**注**：上述文献信息为示例性质，实际引用时建议通过PubMed或Google Scholar以“TRIM45 recombinant protein”为关键词检索核实。TRIM45的研究多集中于其泛素连接酶活性及在神经、免疫系统的功能。
TRIM45. a member of the tripartite motif (TRIM) protein family, is characterized by its conserved N-terminal structure comprising a RING finger domain, B-box motifs, and a coiled-coil region, followed by a C-terminal PRY-SPRY domain. Primarily expressed in the brain and testis, TRIM45 functions as an E3 ubiquitin ligase, modulating protein stability and activity through ubiquitination. It plays regulatory roles in diverse cellular processes, including transcriptional regulation, innate immunity, and stress responses. TRIM45 has been implicated in neurodevelopment and neurodegenerative disorders, with studies suggesting its interaction with protein kinase C (PKC) isoforms to influence neuronal signaling pathways.
Emerging evidence highlights its dual role in disease contexts. In gliomas, TRIM45 acts as a tumor suppressor by inhibiting NF-κB signaling, whereas its downregulation in hepatitis B-infected hepatocytes correlates with enhanced viral replication. Additionally, TRIM45 exhibits antiviral activity against RNA viruses like influenza A and SARS-CoV-2 by targeting viral nucleoproteins for degradation. Paradoxically, it may also promote neuroinflammatory damage in conditions like Parkinson’s disease through excessive proinflammatory cytokine production. Despite these advances, the mechanistic details of TRIM45’s tissue-specific functions and its therapeutic potential remain under investigation, particularly its interplay with autoimmune pathways and stress granule dynamics. Its context-dependent roles underscore the complexity of TRIM protein biology in health and disease.
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