| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC25A42 |
| Uniprot No | Q86VD7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-318 aa |
| 活性数据 | MGNGVKEGPVRLHEDAEAVLSSSVSSKRDHRQVLSSLLPGALAGALAKTAVAPLDRTKIIFQVSSKRFSAKEAFRVLYYTYLNEGFLSLWRGNSATMVRVVPYAAIQFSAHEEYKRILGSYYGFRGEALPPWPRLFAGALAGTTAASLTYPLDLVRARMAVTPKEMYSNIFHVFIRISREEGLKTLYHGFMPTVLGVIPYAGLSFFTYETLKSLHREYSGRRQPYPFERMIFGACAGLIGQSASYPLDVVRRRMQTAGVTGYPRASIARTLRTIVREEGAVRGLYKGLSMNWVKGPIAVGISFTTFDLMQIMLRHLQS |
| 分子量 | 61.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC25A42蛋白的**假设性参考文献示例**(若需真实文献，建议通过PubMed或Google Scholar检索最新研究)：
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1. **文献名称**: *"Recombinant expression and functional characterization of human SLC25A42 as a mitochondrial Coenzyme A transporter"*
**作者**: Wang L., et al.
**摘要**: 研究报道了在HEK293细胞中重组表达人源SLC25A42蛋白，并通过体外线粒体摄取实验证实其作为辅酶A(CoA)转运体的功能，揭示了其对细胞能量代谢的关键作用。
2. **文献名称**: *"Structural insights into human SLC25A42 by cryo-EM: Implications for substrate recognition"*
**作者**: García-Martínez E., et al.
**摘要**: 通过冷冻电镜解析重组人SLC25A42蛋白的三维结构，发现其独特的底物结合口袋，并基于分子对接模拟提出其特异性转运CoA及其他酰基化合物的机制。
3. **文献名称**: *"SLC25A42 variants impair mitochondrial CoA transport and cause neuromuscular disease: Rescue by recombinant protein expression"*
**作者**: Fernández-Vizarra E., et al.
**摘要**: 在患者来源的成纤维细胞中，发现SLC25A42突变导致线粒体CoA转运缺陷，而重组表达野生型蛋白可恢复线粒体代谢功能，为潜在治疗策略提供依据。
4. **文献名称**: *"Optimizing recombinant SLC25A42 production in E. coli for biochemical assays"*
**作者**: Zhang R., et al.
**摘要**: 报道在大肠杆菌系统中高效表达并纯化重组人SLC25A42蛋白的优化方案，验证其体外跨膜转运活性及动力学参数，为药物筛选奠定基础。
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**备注**：以上文献为模拟示例，实际研究中需检索具体数据库(如PubMed DOI)获取真实信息。SLC25A42相关研究多集中于其转运功能及疾病关联，重组蛋白研究可能相对较少，建议结合关键词“recombinant SLC25A42”或“SLC25A42 expression”深入查询。
SLC25A42 is a member of the solute carrier family 25 (SLC25), a group of mitochondrial carrier proteins responsible for transporting metabolites, nucleotides, and cofactors across the mitochondrial inner membrane. As a nuclear-encoded protein, SLC25A42 is synthesized in the cytoplasm and translocated to mitochondria, where it plays a critical role in cellular energy metabolism. It is primarily implicated in the transport of coenzyme A (CoA) and its precursors, such as pantothenate, into mitochondria, supporting essential metabolic pathways, including fatty acid oxidation, the tricarboxylic acid (TCA) cycle, and amino acid metabolism.
Structurally, SLC25A42 contains conserved tripartite repeats and a characteristic mitochondrial carrier domain, which facilitate substrate recognition and transmembrane transport. Dysregulation or mutations in the SLC25A42 gene have been linked to mitochondrial disorders, particularly manifesting as metabolic encephalomyopathies, lactic acidosis, and developmental delays. Recent studies highlight its association with rare genetic conditions like mitochondrial CoA transport deficiency, underscoring its vital role in maintaining mitochondrial CoA homeostasis.
Recombinant human SLC25A42 protein, produced via heterologous expression systems, is widely used in biochemical studies to elucidate transport mechanisms, substrate specificity, and interactions with therapeutic compounds. Its characterization aids in understanding mitochondrial dysfunction and developing targeted therapies for related metabolic diseases. Ongoing research continues to explore its broader regulatory roles in cellular health and disease.
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