| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC25A31 |
| Uniprot No | Q9H0C2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-315 aa |
| 活性数据 | MHREPAKKKAEKRLFDASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMVDCLVRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQFWRWFLANLASGGAAGATSLCVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSDGIAGLYQGFGVSVQGIIVYRASYFGAYDTVKGLLPKPKKTPFLVSFFIAQVVTTCSGILSYPFDTVRRRMMMQSGEAKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGTGGALVLVLYDKIKEFFHIDIGGR |
| 分子量 | 61.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人SLC25A31蛋白的示例参考文献(内容为虚构示例，供参考)：
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1. **文献名称**: *Functional characterization of recombinant human SLC25A31 as a mitochondrial adenine nucleotide transporter*
**作者**: Lee H. et al. (2020)
**摘要**: 本研究在大肠杆菌中重组表达了人SLC25A31蛋白，通过脂质体转运实验验证其具有特异性转运ATP/ADP的能力，并发现其活性受线粒体膜电位调节，提示其在细胞能量代谢中的作用。
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2. **文献名称**: *Structural insights into SLC25A31 by cryo-EM: Implications for nucleotide transport mechanism*
**作者**: Zhang R. et al. (2022)
**摘要**: 利用冷冻电镜解析了重组人SLC25A31的高分辨率结构，揭示其结合ADP的关键氨基酸残基，并通过突变实验证明第138位精氨酸对底物选择性的决定性作用。
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3. **文献名称**: *SLC25A31 knockout induces apoptosis via mitochondrial dysfunction in cancer cells*
**作者**: Gupta S. et al. (2019)
**摘要**: 通过CRISPR技术构建SLC25A31缺陷型癌细胞系，发现其线粒体膜电位下降并激活caspase-3依赖性凋亡通路，表明SLC25A31可能作为肿瘤治疗潜在靶点。
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**提示**：以上为学术示例，实际文献需通过PubMed/Google Scholar以“SLC25A31”或“ANT3”为关键词检索。推荐结合其功能(如线粒体载体、核苷酸转运)及疾病(如癌症、代谢综合征)方向筛选文献。
The recombinant human SLC25A31 protein, also known as adenine nucleotide translocase 4 (ANT4), is a member of the solute carrier family 25 (SLC25) of mitochondrial carrier proteins. Primarily localized in the inner mitochondrial membrane, it facilitates the exchange of ATP and ADP across the membrane, a critical process for cellular energy homeostasis. Unlike other ANT isoforms (ANT1-3), ANT4 exhibits testis-specific expression in humans and plays a vital role in spermatogenesis, particularly during meiosis and post-meiotic stages of sperm development.
Structurally, it shares the conserved tripartite repeat motif of mitochondrial carriers, with six transmembrane α-helices forming a substrate-binding pore. Mutations or dysregulation of SLC25A31 have been linked to male infertility, mitochondrial disorders, and cancer progression due to its dual role in energy metabolism and apoptosis modulation. Recombinant SLC25A31 is often produced in *E. coli* or mammalian expression systems for functional studies, enabling research into its transport kinetics, interaction partners, and pathological mechanisms. Its unique tissue specificity and essential role in germ cell development make it a potential therapeutic target for fertility-related conditions. The gene encoding this protein is located on the X chromosome (Xq24), which may contribute to sex-biased metabolic vulnerabilities.
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