| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC25A3 |
| Uniprot No | Q00325 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-361 aa |
| 活性数据 | MFSSVAHLARANPFNTPHLQLVHDGLGDLRSSSPGPTGQPRRPRNLAAAAVEEYSCEFGSAKYYALCGFGGVLSCGLTHTAVVPLDLVKCRMQVDPQKYKGIFNGFSVTLKEDGVRGLAKGWAPTFLGYSMQGLCKFGFYEVFKVLYSNMLGEENTYLWRTSLYLAASASAEFFADIALAPMEAAKVRIQTQPGYANTLRDAAPKMYKEEGLKAFYKGVAPLWMRQIPYTMMKFACCERTVEALYKFVVPKPRSECSKPEQLVVTFVAGYIAGVFCAIVSHPADSVVSVLNKEKGSSASLVLKRLGFKGVWKGLFARIIMIGTLTALQWFIYDSVKVYFRLPRPPPPEMPESLKKKLGLTQ |
| 分子量 | 65.45 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC25A3蛋白的3篇代表性文献及其摘要概述：
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1. **标题**: *"Functional characterization of recombinant human mitochondrial phosphate carrier protein (SLC25A3) expressed in yeast"*
**作者**: Fiermonte G, et al.
**摘要**: 研究通过酵母表达系统重组人SLC25A3蛋白，证实其作为线粒体磷酸盐载体的功能，发现其具有pH依赖的磷酸氢根(HPO₄²⁻)/质子(H⁺)协同转运活性，并解析了其在不同组织亚型(如心脏和肝脏)中的底物特异性差异。
2. **标题**: *"Structural insights into the human SLC25A3 mitochondrial phosphate carrier by cryo-EM"*
**作者**: Wang Y, et al.
**摘要**: 利用冷冻电镜技术首次解析了重组表达的人SLC25A3蛋白的三维结构，揭示了其独特的跨膜螺旋排列及底物结合位点，为理解磷酸盐转运机制及疾病相关突变的影响提供了结构基础。
3. **标题**: *"SLC25A3 deficiency-associated mitochondrial cardiomyopathy: Rescue by recombinant expression in cellular models"*
**作者**: Bhoj EJ, et al.
**摘要**: 研究通过构建重组人SLC25A3质粒，在患者来源的成纤维细胞中恢复蛋白表达，逆转了线粒体膜电位异常及ATP合成缺陷，证实SLC25A3突变导致婴儿型心肌病的分子机制及潜在基因治疗策略。
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*注：上述文献为示例性质，部分内容基于真实研究整合而成，实际引用需核实原始论文。若需进一步检索具体文献，可基于关键词在PubMed或SciHub等平台获取全文。*
The human SLC25A3 protein, encoded by the *SLC25A3* gene, is a mitochondrial carrier family member localized to the inner mitochondrial membrane. It functions primarily as a phosphate transporter, facilitating the uptake of inorganic phosphate (Pi) into the mitochondrial matrix, which is critical for ATP synthesis. SLC25A3 exists as two isoforms, termed "A" and "B," generated via alternative promoter usage, with isoform A predominantly expressed in heart and skeletal muscle, while isoform B is ubiquitously present. Structurally, it follows the tripartite organization of mitochondrial carriers: six transmembrane helices forming a substrate-binding cavity, with key residues involved in substrate recognition and proton-coupled transport.
Mutations in SLC25A3 are linked to mitochondrial phosphate carrier deficiency, a rare metabolic disorder characterized by lactic acidosis, hypertrophic cardiomyopathy, and muscle hypotonia. Studies suggest that impaired phosphate transport disrupts oxidative phosphorylation, leading to cellular energy deficits. Recombinant SLC25A3 proteins are instrumental in dissecting transport mechanisms, evaluating pathogenic variants, and developing therapeutic strategies. Their production via heterologous expression systems (e.g., *E. coli* or yeast) enables structural and functional analyses, including substrate affinity assays and inhibitor screening. Such research highlights SLC25A3's role in mitochondrial homeostasis and its potential as a target for metabolic disorders.
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