| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RACGAP1 |
| Uniprot No | Q9H0H5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-632aa |
| 氨基酸序列 | MDTMMLNVRN LFEQLVRRVE ILSEGNEVQF IQLAKDFEDF RKKWQRTDHE LGKYKDLLMK AETERSALDV KLKHARNQVD VEIKRRQRAE ADCEKLERQI QLIREMLMCD TSGSIQLSEE QKSALAFLNR GQPSSSNAGN KRLSTIDESG SILSDISFDK TDESLDWDSS LVKTFKLKKR EKRRSTSRQF VDGPPGPVKK TRSIGSAVDQ GNESIVAKTT VTVPNDGGPI EAVSTIETVP YWTRSRRKTG TLQPWNSDST LNSRQLEPRT ETDSVGTPQS NGGMRLHDFV SKTVIKPESC VPCGKRIKFG KLSLKCRDCR VVSHPECRDR CPLPCIPTLI GTPVKIGEGM LADFVSQTSP MIPSIVVHCV NEIEQRGLTE TGLYRISGCD RTVKELKEKF LRVKTVPLLS KVDDIHAICS LLKDFLRNLK EPLLTFRLNR AFMEAAEITD EDNSIAAMYQ AVGELPQANR DTLAFLMIHL QRVAQSPHTK MDVANLAKVF GPTIVAHAVP NPDPVTMLQD IKRQPKVVER LLSLPLEYWS QFMMVEQENI DPLHVIENSN AFSTPQTPDI KVSLLGPVTT PEHQLLKTPS SSSLSQRVRS TLTKNTPRFG SKSKSATNLG RQGNFFASPM LK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RACGAP1重组蛋白的参考文献示例(注：以下文献为虚构示例，仅供格式参考)：
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1. **"RACGAP1 recombinant protein regulates cytokinesis via RhoA signaling in vitro"**
*作者：Chen L, et al. (2022)*
**摘要**：本研究通过大肠杆菌表达系统纯化RACGAP1重组蛋白，发现其在体外显著增强RhoA的GTP酶活性，调控细胞分裂过程中收缩环的形成，提示其在胞质分裂中的关键作用。
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2. **"Functional analysis of recombinant RACGAP1 in breast cancer cell migration"**
*作者：Wang X, et al. (2021)*
**摘要**：利用哺乳动物细胞表达的重组RACGAP1蛋白，证实其通过调控Rac1信号通路促进乳腺癌细胞迁移，为靶向RACGAP1的癌症治疗提供实验依据。
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3. **"Crystal structure of the RACGAP1 C-terminal domain and implications for dimerization"**
*作者：Kimura T, et al. (2020)*
**摘要**：首次解析RACGAP1重组蛋白C端结构域晶体结构，揭示其二聚化机制及其与微管结合蛋白相互作用的功能基序。
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4. **"Development of a high-throughput assay for RACGAP1 GTPase-activating activity using recombinant protein"**
*作者：Gomez R, et al. (2019)*
**摘要**：建立基于重组RACGAP1蛋白的高通量筛选平台，用于鉴定调控Rac/Rho信号通路的小分子抑制剂，为药物开发提供工具。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“RACGAP1 recombinant protein”获取最新研究。
Rac GTPase-activating protein 1 (RACGAP1) is a key regulator of cellular processes involving Rho family GTPases, particularly Rac and Cdc42. As a member of the GTPase-activating protein (GAP) family, RACGAP1 accelerates the hydrolysis of GTP to GDP on these signaling molecules, thereby modulating their activity in cytoskeletal reorganization, cell division, and migration. Structurally, RACGAP1 contains a conserved GAP domain critical for its enzymatic function and coiled-coil regions that facilitate protein-protein interactions. Notably, it forms a heterodimeric complex with ECT2 (Epithelial Cell Transforming 2) during cytokinesis, playing an essential role in the formation of the contractile ring and completion of cell division.
Recombinant RACGAP1 proteins are engineered using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. These proteins often include affinity tags (e.g., His or FLAG) for purification and detection. Recombinant variants enable detailed biochemical studies, such as analyzing GAP activity, mapping interaction domains, or screening inhibitors. In cancer research, RACGAP1 is of interest due to its overexpression in tumors (e.g., breast, lung, and gastric cancers), where it promotes invasion, metastasis, and chemoresistance. Its role in cytokinesis also links it to polyploidy and genomic instability when dysregulated.
Beyond basic research, recombinant RACGAP1 supports drug discovery by serving as a target or tool to study pathways involving Rho GTPases. Its dynamic expression during the cell cycle (peaking at G2/M phase) further underscores its therapeutic potential. Studies using knockdown models highlight its functional importance, making recombinant RACGAP1 invaluable for dissecting mechanisms in both normal physiology and disease contexts.
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