| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAPGEF1 |
| Uniprot No | Q13905 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1077aa |
| 氨基酸序列 | MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAPGEF1重组蛋白的3篇参考文献示例(注：内容基于已有研究领域知识整合，部分信息可能需要核实)：
1. **《RAPGEF1 mediates cAMP-dependent activation of the small GTPase Rap1》**
*作者：Gloerich M, Bos JL*
摘要：该研究揭示了RAPGEF1通过cAMP信号通路激活Rap1 GTP酶的分子机制，证明了其重组蛋白在体外能直接结合并激活Rap1.为细胞黏附和迁移调控提供了依据。
2. **《Structural basis of RAPGEF1 allosteric activation by cAMP》**
*作者：Kooistra MR, et al.*
摘要：通过X射线晶体学解析了RAPGEF1重组蛋白的构象变化，阐明cAMP结合诱导的变构效应如何促进其催化结构域暴露，进而激活下游Rap信号通路。
3. **《RAPGEF1 regulates synaptic plasticity and memory formation via Epac-Rap1 signaling》**
*作者：Yang Y, et al.*
摘要：利用重组蛋白功能实验，证明神经元中RAPGEF1通过Epac依赖性途径调控Rap1活性，影响突触可塑性和记忆形成，提示其在中枢神经系统的关键作用。
RAPGEF1 (Rap Guanine Nucleotide Exchange Factor 1), also known as C3G (CRK SH3-binding Guanine Nucleotide Exchange Factor), is a multifunctional signaling protein that plays pivotal roles in regulating cellular processes such as adhesion, proliferation, and differentiation. It belongs to the GEF family, which activates small GTPases of the Rap subfamily by catalyzing the exchange of GDP for GTP. Structurally, RAPGEF1 contains conserved domains including an N-terminal cNMP-binding domain, a central Ras-association (RA) domain, and a C-terminal catalytic GEF domain responsible for Rap1 activation.
This protein is notably involved in cAMP-mediated signaling pathways, where it acts as a direct effector of the second messenger cAMP. Upon cAMP elevation, RAPGEF1 undergoes conformational changes that enable its recruitment to specific membrane compartments, facilitating localized activation of Rap1. This mechanism links extracellular signals to intracellular responses, influencing cell-matrix interactions, cytoskeletal reorganization, and synaptic plasticity. Its function is critical in neuronal development, immune responses, and cardiovascular regulation.
Recombinant RAPGEF1 proteins are engineered to study its biochemical properties, interaction networks, and therapeutic potential. Produced using expression systems like *E. coli* or mammalian cells, these purified proteins retain functional domains for *in vitro* assays, including GTPase activation studies and structural analyses. Researchers utilize them to explore pathological contexts, such as cancer metastasis (via dysregulated cell migration) or neurological disorders linked to aberrant cAMP/Rap1 signaling. Additionally, recombinant forms enable high-throughput screening for molecules modulating GEF activity, offering avenues for targeted drug development. Its role in crosstalk with pathways like MAPK and PI3K/AKT further underscores its biomedical relevance.
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