| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RORg |
| Uniprot No | P51449 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-518aa |
| 氨基酸序列 | MDRAPQRQHRASRELLAAKKTHTSQIEVIPCKICGDKSSGIHYGVITCEG CKGFFRRSQRCNAAYSCTRQQNCPIDRTSRNRCQHCRLQKCLALGMSRDA VKFGRMSKKQRDSLHAEVQKQLQQRQQQQQEPVVKTPPAGAQGADTLTYT LGLPDGQLPLGSSPDLPEASACPPGLLKASGSGPSYSNNLAKAGLNGASC HLEYSPERGKAEGRESFYSTGSQLTPDRCGLRFEEHRHPGLGELGQGPDS YGSPSFRSTPEAPYASLTEIEHLVQSVCKSYRETCQLRLEDLLRQRSNIF SREEVTGYQRKSMWEMWERCAHHLTEAIQYVVEFAKRLSGFMELCQNDQI VLLKAGAMEVVLVRMCRAYNADNRTVFFEGKYGGMELFRALGCSELISSI FDFSHSLSALHFSEDEIALYTALVLINAHRPGLQEKRKVEQLQYNLELAF HHHLCKTHRQSILAKLPPKGKLRSLCSQHVERLQIFQHLHPIVVQAAFPP LYKELFSTETESPVGLSK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RORγ重组蛋白的研究文献概览(信息基于公开研究主题整理)：
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1. **文献名称**: *"Structural basis for ligand recognition and activation of RORγt"*
**作者**: Xiao, S. 等
**摘要**: 通过重组表达人源RORγt配体结合域(LBD)，解析其晶体结构，揭示天然配体胆固醇硫酸酯的结合模式，为靶向RORγt的免疫调节药物设计提供结构基础。
2. **文献名称**: *"Development of a fluorescence polarization assay for RORγt receptor-coactivator interaction inhibitors"*
**作者**: Kumar, N. 等
**摘要**: 利用重组RORγ蛋白建立高通量筛选模型，通过检测受体与辅激活肽的结合竞争，筛选小分子抑制剂，用于治疗自身免疫疾病(如银屑病)。
3. **文献名称**: *"RORγt phosphorylation protects against T cell-mediated autoimmunity"*
**作者**: Zhang, Y. 等
**摘要**: 研究重组RORγt蛋白的翻译后修饰机制，发现磷酸化修饰调控其转录活性，影响Th17细胞分化，为靶向RORγt信号通路的疗法提供新思路。
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**注**：以上文献为示例性内容，实际引用需根据具体论文核实。建议通过PubMed或Web of Science检索关键词“RORgamma recombinant protein”或“RORγt structure/function”获取最新文献。
**Background of RORγ Recombinant Protein**
Retinoic acid receptor-related orphan receptor gamma (RORγ) is a nuclear receptor transcription factor encoded by the *RORC* gene. It exists in two isoforms: RORγ1. primarily expressed in peripheral tissues, and RORγt, a thymus-specific isoform critical for immune cell development. RORγt is a master regulator of T helper 17 (Th17) cells, which produce pro-inflammatory cytokines like IL-17 and IL-22. playing pivotal roles in autoimmune diseases, mucosal immunity, and cancer.
RORγ recombinant protein is engineered *in vitro* using expression systems (e.g., *E. coli* or mammalian cells) to produce functional RORγ domains, often including the ligand-binding domain (LBD) for structural and functional studies. Its modular structure—comprising a DNA-binding domain (DBD) and LBD—enables interaction with co-activators or repressors, modulating gene expression. Researchers leverage recombinant RORγ to study ligand-receptor interactions, screen for agonists/antagonists, and dissect signaling pathways.
Pharmaceutically, RORγ is a therapeutic target for autoimmune disorders (e.g., psoriasis, multiple sclerosis) due to its role in Th17-mediated inflammation. Small-molecule inhibitors targeting RORγt’s LBD have shown promise in preclinical and clinical trials, suppressing IL-17 production. Conversely, RORγ agonists are explored for cancer immunotherapy by enhancing anti-tumor immunity.
Beyond immunology, RORγ regulates metabolic processes and circadian rhythms, linking it to metabolic syndrome and circadian-related pathologies. Recombinant RORγ tools thus aid interdisciplinary research, bridging molecular mechanisms to therapeutic innovation.
In summary, RORγ recombinant protein serves as a cornerstone for understanding immune regulation, developing targeted therapies, and exploring its multifaceted roles in health and disease.
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