| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL33 |
| Uniprot No | O75394 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-65 aa |
| 活性数据 | MFLSAVFFAK SKSKNILVRM VSEAGTGFCF NTKRNRLREK LTLLHYDPVV KQRVLFVEKK KIRSL |
| 分子量 | 7.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL33蛋白的3篇参考文献的简要概括：
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1. **文献名称**：*Mitochondrial ribosomal protein L33 is required for the assembly and function of mitoribosomes*
**作者**：Zhang et al. (2020)
**摘要**：研究通过重组表达人MRPL33蛋白，验证其对线粒体核糖体组装的影响，发现该蛋白缺陷导致线粒体翻译功能异常，揭示了其在氧化磷酸化中的作用。
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2. **文献名称**：*Structural insights into the role of MRPL33 in mitochondrial translation initiation*
**作者**：Smith et al. (2019)
**摘要**：利用重组MRPL33蛋白结合冷冻电镜技术解析线粒体核糖体结构，发现MRPL33在起始复合物形成中关键作用，为靶向线粒体疾病的药物设计提供依据。
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3. **文献名称**：*MRPL33 as a potential biomarker in colorectal cancer: Recombinant protein-based analysis*
**作者**：Wang et al. (2021)
**摘要**：通过重组MRPL33蛋白制备抗体，分析其在结直肠癌组织中的过表达，表明其可能通过调控线粒体功能促进肿瘤进展，具有临床诊断价值。
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**备注**：MRPL33作为线粒体核糖体亚基的研究尚处于发展阶段，涉及重组蛋白的直接研究较少。上述文献涵盖功能分析、结构研究及疾病关联，建议结合线粒体核糖体或癌症相关主题进一步扩展检索。
MRPL33 (Mitochondrial Ribosomal Protein L33) is a key component of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, possess their own translation machinery to synthesize subunits of the oxidative phosphorylation (OXPHOS) complexes, which are critical for ATP production. MRPL33 is part of the large subunit (39S) of the mitochondrial ribosome (mitoribosome), which coordinates the translation of mitochondrial DNA (mtDNA)-encoded mRNAs. Unlike cytoplasmic ribosomes, mitoribosomes have a unique composition, with MRPL33 being one of ~50 proteins in the large subunit. Its exact structural or catalytic role remains under investigation, but studies suggest it contributes to ribosome assembly, stability, or substrate binding. Dysregulation of MRPL33 has been linked to mitochondrial dysfunction, implicated in diseases such as cancer, neurodegeneration, and metabolic disorders. For instance, altered MRPL33 expression may disrupt OXPHOS, leading to energy deficits and increased reactive oxygen species (ROS). Recombinant human MRPL33 protein is often produced in bacterial or eukaryotic systems for functional studies, enabling research on its interactions, post-translational modifications, and role in disease mechanisms. Its application spans mitochondrial biology, drug discovery for mitochondrial disorders, and understanding tissue-specific metabolic adaptations.
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