| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL24 |
| Uniprot No | Q96A35 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 10-216 aa |
| 活性数据 | A SKVTLPPHYR YGMSPPGSVA DKRKNPPWIR RRPVVVEPIS DEDWYLFCGD TVEILEGKDA GKQGKVVQVI RQRNWVVVGG LNTHYRYIGK TMDYRGTMIP SEAPLLHRQV KLVDPMDRKP TEIEWRFTEA GERVRVSTRS GRIIPKPEFP RADGIVPETW IDGPKDTSVE DALERTYVPC LKTLQEEVME AMGIKETRKY KKVYWY |
| 分子量 | 24.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL24蛋白的模拟参考文献示例(实际文献需通过学术数据库核实)：
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1. **文献名称**: *"Structural insights into the human mitochondrial ribosome assembly: Role of MRPL24 in large subunit biogenesis"*
**作者**: Johnson A. et al.
**摘要**: 该研究通过冷冻电镜解析人线粒体核糖体大亚基结构，发现重组表达的MRPL24蛋白对亚基组装至关重要，并揭示其与16S rRNA的相互作用位点。
2. **文献名称**: *"MRPL24 deficiency disrupts mitochondrial translation and promotes cancer cell apoptosis via OXPHOS impairment"*
**作者**: Chen L. et al.
**摘要**: 利用重组MRPL24进行功能恢复实验，发现其缺失导致线粒体翻译缺陷，呼吸链复合物减少，进而诱导癌细胞凋亡，提示其作为潜在抗癌靶点。
3. **文献名称**: *"Recombinant MRPL24 expression rescues mitochondrial dysfunction in patient-derived fibroblasts"*
**作者**: Wang Y. et al.
**摘要**: 研究在MRPL24突变导致的线粒体疾病模型细胞中，通过重组MRPL24蛋白回补实验，部分恢复线粒体蛋白合成能力，验证其病理相关性。
4. **文献名称**: *"Interactome mapping of MRPL24 reveals its binding partners in mitochondrial translation regulation"*
**作者**: Müller R. et al.
**摘要**: 采用重组MRPL24进行蛋白质互作实验，鉴定其与线粒体翻译延伸因子mtEF-Tu的相互作用，提示其在翻译延伸过程中的调控作用。
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**注意事项**：以上文献为模拟示例，实际文献需通过PubMed、Web of Science等平台检索关键词(如“MRPL24 recombinant”“mitochondrial ribosome”)获取。真实研究多聚焦于MRPL24的线粒体翻译功能、疾病关联(如癌症、罕见病)及结构生物学分析。
**Recombinant Human MRPL24 Protein: Background and Significance**
The mitochondrial ribosomal protein L24 (MRPL24) is a key component of the large subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Mitochondria, the cellular powerhouses, rely on their own ribosomes to translate mitochondrial DNA (mtDNA)-encoded genes, which are critical for oxidative phosphorylation (OXPHOS) and ATP production. MRPL24 plays a structural and functional role in ribosome assembly and ensures the proper translation of mtDNA-encoded subunits of the electron transport chain (ETC) complexes.
As a recombinant protein, MRPL24 is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell lines, followed by purification. This enables large-scale study of its molecular interactions, structural features, and role in mitochondrial dysfunction. MRPL24 mutations or dysregulation have been linked to mitochondrial disorders, metabolic syndromes, and certain cancers, underscoring its importance in cellular energy homeostasis. Recombinant MRPL24 serves as a tool for investigating mitochondrial translation defects, modeling diseases, and screening potential therapeutics targeting mitochondrial pathways. Its study contributes to understanding how mitochondrial ribosome dysfunction impacts human health and aging.
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