| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL32 |
| Uniprot No | Q9BYC8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-188 aa |
| 活性数据 | MALAMLVLVVSPWSAARGVLRNYWERLLRKLPQSRPGFPSPPWGPALAVQGPAMFTEPANDTSGSKENSSLLDSIFWMAAPKNRRTIEVNRCRRRNPQKLIKVKNNIDVCPECGHLKQKHVLCAYCYEKVCKETAEIRRQIGKQEGGPFKAPTIETVVLYTGETPSEQDQGKRIIERDRKRPSWFTQN |
| 分子量 | 47.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL32蛋白的3篇代表性文献概述(基于虚拟数据整理，仅供参考)：
1. **Title**: "Molecular Cloning and Functional Characterization of Human MRPL32. a Mitochondrial Ribosomal Protein Involved in Apoptosis Regulation"
**Authors**: Jia L, et al.
**Summary**: 本研究克隆了人MRPL32基因，并在HEK293细胞中重组表达了该蛋白。实验表明MRPL32与线粒体核糖体大亚基组装相关，并可能通过调控Bcl-2家族蛋白参与细胞凋亡通路。
2. **Title**: "Structural Insights into MRPL32's Role in Mitochondrial Translation Machinery"
**Authors**: Smith RJ, et al.
**Summary**: 通过X射线晶体学解析了重组MRPL32蛋白的三维结构，揭示了其与线粒体rRNA结合的特定结构域，为理解其在线粒体蛋白质合成中的功能提供了结构基础。
3. **Title**: "MRPL32 Deficiency Impairs Mitochondrial Respiratory Chain Assembly and Causes Metabolic Dysregulation"
**Authors**: Lee S, Wang Y.
**Summary**: 利用CRISPR/Cas9敲除细胞模型，发现MRPL32缺失会导致线粒体复合物I和III组装异常，进而引发氧化磷酸化功能障碍，提示其在能量代谢中的关键作用。
(注：上述文献为模拟生成，实际研究中建议通过PubMed或Web of Science检索具体文献。)
Human MRPL32 (Mitochondrial Ribosomal Protein L32) is a component of the mitochondrial ribosome, specifically the large (39S) subunit, which plays a critical role in mitochondrial protein synthesis. Encoded by the nuclear genome and imported into mitochondria, MRPL32 is part of the highly conserved L32 ribosomal protein family. It contributes to ribosome assembly, structural stability, and the translation of mitochondrial DNA-encoded mRNAs, which are essential for oxidative phosphorylation (OXPHOS) and cellular energy production.
Dysregulation of MRPL32 has been linked to mitochondrial dysfunction, implicated in metabolic disorders, neurodegenerative diseases, and cancer. Studies suggest that altered MRPL32 expression affects mitochondrial respiratory chain activity, potentially disrupting ATP synthesis and promoting disease progression.
Recombinant MRPL32 protein, produced via bacterial or eukaryotic expression systems, is widely used to investigate its structural interactions within the ribosome, functional roles in mitochondrial translation, and pathological mechanisms. Its purification often involves affinity chromatography and tag-based methods. Research applications include in vitro ribosome reconstitution assays, antibody development, and screening for mitochondrial-targeted therapies.
Understanding MRPL32's molecular mechanisms provides insights into mitochondrial biology and therapeutic strategies for diseases associated with energy metabolism defects.
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