| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL22 |
| Uniprot No | Q9NWU5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 41-206 aa |
| 活性数据 | ISRKWEKKNK IVYPPQLPGE PRRPAEIYHC RRQIKYSKDK MWYLAKLIRG MSIDQALAQL EFNDKKGAKI IKEVLLEAQD MAVRDHNVEF RSNLYIAEST SGRGQCLKRI RYHGRGRFGI MEKVYCHYFV KLVEGPPPPP EPPKTAVAHA KEYIQQLRSR TIVHTL |
| 分子量 | 23.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL22蛋白的示例参考文献(基于模拟数据，建议通过学术数据库验证具体内容)：
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1. **文献名称**: *Structural and functional analysis of MRPL22 in mitochondrial ribosome assembly*
**作者**: Smith A et al.
**摘要**: 研究了人源MRPL22蛋白在线粒体核糖体组装中的结构作用，利用重组表达的MRPL22结合冷冻电镜技术解析其与线粒体rRNA的互作机制。
2. **文献名称**: *MRPL22 deficiency induces mitochondrial dysfunction and apoptosis in cancer cells*
**作者**: Chen L et al.
**摘要**: 通过重组MRPL22基因敲低实验，发现其缺失导致线粒体翻译障碍、活性氧积累，并激活细胞凋亡通路，提示其在肿瘤代谢中的潜在调控作用。
3. **文献名称**: *Expression and purification of recombinant human MRPL22 for antibody development*
**作者**: Kumar R et al.
**摘要**: 报道了在大肠杆菌系统中高效表达可溶性重组人MRPL22蛋白的方法，并利用纯化产物成功制备了特异性抗体，用于后续临床样本检测。
4. **文献名称**: *The role of MRPL22 in mitoribosome biogenesis: Insights from yeast homolog studies*
**作者**: Garcia M et al.
**摘要**: 比较了人源MRPL22与其酵母同源蛋白的功能保守性，通过重组蛋白互补实验验证其在核糖体亚基稳定性中的关键作用。
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**注意事项**：
1. 以上文献为示例，实际研究可能需结合具体数据库(如PubMed、Web of Science)检索最新成果。
2. MRPL22相关研究较少，建议扩大关键词范围(如“线粒体核糖体蛋白”或疾病关联名称)。
3. 重组蛋白表达技术可参考通用线粒体蛋白制备文献作为补充。
Mitochondrial ribosomal protein L22 (MRPL22) is a component of the large subunit of the mitochondrial ribosome (mitoribosome), which is essential for protein synthesis within mitochondria. Encoded by nuclear DNA, MRPL22 is synthesized in the cytoplasm and transported to the mitochondrial matrix, where it integrates into the mitoribosome structure. This protein plays a critical role in assembling and stabilizing the mitoribosome, facilitating the translation of mitochondrial DNA (mtDNA)-encoded genes, particularly those involved in oxidative phosphorylation (OXPHOS) complexes. These complexes are vital for cellular energy production through ATP synthesis.
MRPL22’s structure includes conserved motifs common to mitochondrial ribosomal proteins, though its precise functional domains remain under investigation. Dysregulation of MRPL22 has been linked to mitochondrial dysfunction, implicated in disorders such as cancer, neurodegenerative diseases, and metabolic syndromes. For instance, abnormal MRPL22 expression may disrupt OXPHOS, leading to impaired energy metabolism and increased oxidative stress. Recombinant MRPL22 is typically produced using bacterial or mammalian expression systems, enabling studies on its biochemical properties, interactions, and role in mitochondrial translation. Research on this protein enhances understanding of mitochondrial pathophysiology and potential therapeutic strategies targeting energy metabolism disorders. Its study also contributes to insights into evolutionary conservation of ribosomal machinery across species.
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