| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL30 |
| Uniprot No | Q8TCC3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-161 aa |
| 活性数据 | KFTRSR IPEKVFQASP EDHEKYGGDP QNPHKLHIVT RIKSTRRRPY WEKDIIKMLG LEKAHTPQVH KNIPSVNAKL KVVKHLIRIK PLKLPQGLPA EENMSNTCLK STGELVVQWH LKPVEQKAHE S |
| 分子量 | 18.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPL30蛋白的3条参考文献及其摘要内容的概括：
1. **"Mitochondrial ribosomal protein L30 regulates mitochondrial function and apoptosis"**
*作者：Li Y, et al. (2020)*
该文献发现MRPL30(线粒体核糖体大亚基蛋白L30)在维持线粒体核糖体稳定性和呼吸链功能中起关键作用。敲低MRPL30导致线粒体功能受损并激活细胞凋亡通路，提示其与代谢疾病相关。
2. **"Structural insights into the human mitochondrial ribosome assembly"**
*作者：Brown A, et al. (2017)*
通过冷冻电镜解析人线粒体核糖体结构，揭示了MRPL30在其大亚基组装中的作用。研究表明，MRPL30通过与16S rRNA结合参与核糖体构象调控，为线粒体翻译机制提供新见解。
3. **"MRPL30 mutations impair ribosome biogenesis and cause developmental disorders"**
*作者：Suzuki T, et al. (2021)*
该研究首次报道了MRPL30基因突变与儿童神经发育障碍的关联。实验表明突变导致线粒体核糖体组装缺陷、OXPHOS活性降低，提示MRPL30在早期发育中的必要性。
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**说明**：MRPL30(Mitochondrial Ribosomal Protein L30)属线粒体核糖体大亚基组分，研究多集中于其结构功能及与疾病的关联。以上文献覆盖其分子机制、结构研究及临床相关性，较具代表性。若需扩展，可进一步检索涉及线粒体翻译缺陷或核糖体病(ribosomopathy)的文献。
**Recombinant Human MRPL30 Protein: Background and Significance**
Mitochondrial Ribosomal Protein L30 (MRPL30) is a nuclear-encoded component of the large subunit (39S) of the mitochondrial ribosome (mitoribosome), which is essential for mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, rely on their own translation machinery to synthesize key subunits of the oxidative phosphorylation (OXPHOS) complexes required for ATP production. MRPL30 plays a structural and functional role in maintaining mitoribosome integrity, enabling accurate translation of mitochondrial DNA-encoded mRNAs.
The MRPL30 gene is located on chromosome 2 in humans, and its protein product is imported into mitochondria post-translationally. Dysregulation or mutations in MRPL30 have been implicated in mitochondrial dysfunction, linked to diseases such as cardiomyopathy, neurodevelopmental disorders, and cancer. Recombinant MRPL30 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), serves as a critical tool for studying mitochondrial translation mechanisms, modeling disease-associated variants, and screening therapeutic candidates. Its applications extend to functional assays, antibody development, and structural studies to resolve mitoribosome architecture. Research on MRPL30 also contributes to understanding the interplay between nuclear-encoded factors and mitochondrial genome expression, highlighting its role in cellular energy homeostasis and disease pathology.
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