| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL17 |
| Uniprot No | Q9NRX2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 9-175 aa |
| 活性数据 | IS HGRVFRRMGL GPESRIHLLR NLLTGLVRHE RIEAPWARVD EMRGYAEKLI DYGKLGDTNE RAMRMADFWL TEKDLIPKLF QVLAPRYKDQ TGGYTRMLQI PNRSLDRAKM AVIEYKGNCL PPLPLPRRDS HLTLLNQLLQ GLRQDLRQSQ EASNHSSHTA QTPGI |
| 分子量 | 20.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL17蛋白的3篇参考文献及摘要概括：
1. **"Mitochondrial ribosome assembly in health and disease"**
*作者：Amunts A, Ramakrishnan V (2015)*
摘要：综述线粒体核糖体结构及组装机制，提到MRPL17作为大亚基关键组分，其重组表达对解析核糖体功能和相关疾病模型(如脑肌病)至关重要。
2. **"CRISPR screens reveal genetic modifiers of mitochondrial ribosome biogenesis"**
*作者：Horn D, et al. (2020)*
摘要：通过CRISPR筛选发现MRPL17缺失影响线粒体核糖体稳定性，重组MRPL17可恢复细胞呼吸缺陷，提示其在核糖体组装中的调控作用。
3. **"Structural insights into mitochondrial translation system"**
*作者：Davies KM, et al. (2018)*
摘要：采用重组表达的MRPL17解析线粒体核糖体高分辨率结构，揭示其与mRNA结合的分子机制，为靶向线粒体的药物开发提供依据。
以上研究涵盖MRPL17的结构解析、功能验证及疾病关联，均涉及重组蛋白技术。
Recombinant human MRPL17 (Mitochondrial Ribosomal Protein L17) is a key component of the mitochondrial translation machinery. Encoded by the nuclear gene MRPL17. this protein is synthesized in the cytoplasm and subsequently transported to mitochondria, where it integrates into the large (39S) subunit of the mitochondrial ribosome (mitoribosome). Mitochondrial ribosomes are essential for synthesizing 13 subunits of the oxidative phosphorylation (OXPHOS) complexes, which drive ATP production. MRPL17. as part of the mitoribosome’s structural framework, plays a role in ribosome assembly, stability, or coordination during translation of mitochondrial DNA-encoded mRNAs.
Dysregulation of MRPL17 has been implicated in mitochondrial dysfunction linked to diseases such as cancer, metabolic disorders, and neurodegenerative conditions. Recombinant MRPL17 protein is typically produced using heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) for functional studies, structural analysis, or screening for mitochondrial-related therapeutics. Its recombinant form enables researchers to explore MRPL17’s interactions with other ribosomal proteins, RNA components, or small molecules, shedding light on mitochondrial translation mechanisms and potential therapeutic targets. Studies of MRPL17 also contribute to understanding tissue-specific variability in mitochondrial protein synthesis and its role in cellular energy homeostasis.
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