| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL18 |
| Uniprot No | Q9H0U6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-180 aa |
| 活性数据 | MALRSRFWGLFSVCRNPGCRFAALSTSSEPAAKPEVDPVENEAVAPEFTNRNPRNLELLSVARKERGWRTVFPSREFWHRLRVIRTQHHVEALVEHQNGKVVVSASTREWAIKKHLYSTRNVVACESIGRVLAQRCLEAGINFMVYQPTPWEAASDSMKRLQSAMTEGGVVLREPQRIYE |
| 分子量 | 47 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL18蛋白的参考文献示例(内容基于公开研究归纳，需进一步验证)：
1. **《Mitochondrial Ribosomal Protein L18 is Essential for Embryonic Development and Cardiac Function》**
- **作者**: Smith A, et al.
- **摘要**: 研究通过基因敲除小鼠模型发现，MRPL18缺失导致线粒体翻译缺陷及胚胎致死。重组人MRPL18蛋白在体外成功表达并验证其在线粒体核糖体组装中的作用，为心肌病机制提供依据。
2. **《Structural Insights into the Role of MRPL18 in Mitochondrial Ribosome Assembly》**
- **作者**: Chen B, et al.
- **摘要**: 利用冷冻电镜解析重组人MRPL18蛋白的三维结构，揭示其与16S rRNA的结合域，阐明其在核糖体大亚基组装中的关键作用，提示与线粒体疾病相关突变位点。
3. **《MRPL18 Mutation Impairs Respiratory Chain Complexes and Induces Metabolic Dysfunction》**
- **作者**: Wang Y, et al.
- **摘要**: 报道MRPL18基因突变患者存在呼吸链复合体I/III缺陷，通过重组表达野生型MRPL18蛋白恢复细胞模型中线粒体功能，证实其与能量代谢障碍的直接关联。
4. **《Recombinant Expression and Functional Characterization of Human MRPL18 in Yeast》**
- **作者**: Lee S, et al.
- **摘要**: 在酵母系统中高效表达重组人MRPL18蛋白，发现其可部分挽救线粒体核糖体缺陷表型，验证其进化保守性并建立跨物种功能研究平台。
**注**: 上述文献为示例性质，实际引用需通过PubMed等数据库检索最新文献，可使用关键词“MRPL18”、“recombinant”、“mitochondrial ribosome”进一步筛选。
MRPL18 (Mitochondrial Ribosomal Protein L18) is a key component of the mitochondrial large ribosomal subunit, essential for mitochondrial protein synthesis. Encoded by nuclear DNA, it is imported into mitochondria, where it participates in assembling the mitochondrial ribosome. This ribosome specializes in translating the 13 mitochondrial DNA-encoded proteins, all critical subunits of the electron transport chain (ETC) complexes required for oxidative phosphorylation (OXPHOS). As mitochondria lack a full protein synthesis system, nuclear-encoded proteins like MRPL18 are vital for maintaining mitochondrial translational machinery and energy production.
Recombinant MRPL18 refers to the protein produced in vitro using expression systems (e.g., E. coli, yeast, or mammalian cells) for functional studies. Its recombinant form enables research into mitochondrial ribosome structure, respiratory chain deficiencies, and diseases linked to mitochondrial dysfunction, such as metabolic disorders, neurodegenerative conditions, and cancer. Mutations in MRPL18 or disrupted ribosomal assembly can impair mitochondrial translation, leading to ETC defects and cellular energy deficits. Current studies focus on its structural interactions within the ribosome, regulatory roles in OXPHOS, and potential therapeutic targets. The recombinant protein also aids in antibody development for diagnostic applications. While much remains unknown, MRPL18 exemplifies the intricate crosstalk between nuclear-encoded factors and mitochondrial health.
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