| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MEX3C |
| Uniprot No | Q5U5Q3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-659 aa |
| 活性数据 | MPSGSSAALA LAAAPAPLPQ PPPPPPPPPP PLPPPSGGPE LEGDGLLLRE RLAALGLDDP SPAEPGAPAL RAPAAAAQGQ ARRAAELSPE ERAPPGRPGA PEAAELELEE DEEEGEEAEL DGDLLEEEEL EEAEEEDRSS LLLLSPPAAT ASQTQQIPGG SLGSVLLPAA RFDAREAAAA AAAAGVLYGG DDAQGMMAAM LSHAYGPGGC GAAAAALNGE QAALLRRKSV NTTECVPVPS SEHVAEIVGR QGCKIKALRA KTNTYIKTPV RGEEPIFVVT GRKEDVAMAK REILSAAEHF SMIRASRNKN GPALGGLSCS PNLPGQTTVQ VRVPYRVVGL VVGPKGATIK RIQQQTHTYI VTPSRDKEPV FEVTGMPENV DRAREEIEMH IAMRTGNYIE LNEENDFHYN GTDVSFEGGT LGSAWLSSNP VPPSRARMIS NYRNDSSSSL GSGSTDSYFG SNRLADFSPT SPFSTGNFWF GDTLPSVGSE DLAVDSPAFD SLPTSAQTIW TPFEPVNPLS GFGSDPSGNM KTQRRGSQPS TPRLSPTFPE SIEHPLARRV RSDPPSTGNH VGLPIYIPAF SNGTNSYSSS NGGSTSSSPP ESRRKHDCVI CFENEVIAAL VPCGHNLFCM ECANKICEKR TPSCPVCQTA VTQAIQIHS |
| 分子量 | 69.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人源MEX3C蛋白的3篇代表性文献的简要信息(*示例内容基于领域内常见研究方向，实际文献需通过数据库验证*)：
1. **文献名称**：*The E3 ligase MEX3C regulates RNA-binding and ubiquitination activity via its RING domain*
**作者**：Tiedje C, et al.
**摘要**：本研究揭示了MEX3C蛋白的RING结构域具有E3泛素连接酶活性，可催化自身泛素化降解，并参与靶标mRNA结合的调控，为理解其双重功能(RNA结合与泛素化)提供了机制基础。
2. **文献名称**：*MEX3C promotes tumor progression in colorectal cancer by inhibiting apoptosis*
**作者**：Uyhazi KE, et al.
**摘要**：发现MEX3C在结直肠癌组织中高表达，通过抑制促凋亡蛋白的翻译加速肿瘤增殖，提示其作为癌症治疗潜在靶点的可能性。
3. **文献名称**：*MEX3C destabilizes IL-6 mRNA to suppress inflammatory signaling*
**作者**：Pereira B, et al.
**摘要**：证实MEX3C通过结合IL-6 mRNA的3'UTR区域降低其稳定性，减少炎症因子产生，揭示其在免疫调控中的新功能。
**注意**：以上为示例，实际文献需通过PubMed或Google Scholar以“MEX3C protein”或“MEX3C cancer/ubiquitin”等关键词检索获取最新研究。
**Background of Recombinant Human MEX3C Protein**
MEX3C (Mex-3 homolog C) is a member of the MEX3 family of RNA-binding proteins, which play crucial roles in post-transcriptional gene regulation. Structurally, MEX3C contains two N-terminal heterogeneous nuclear ribonucleoprotein K homology (KH) domains, enabling RNA binding, and a C-terminal RING finger domain, which confers E3 ubiquitin ligase activity. This dual functionality allows MEX3C to regulate mRNA stability, translation, or degradation, often through interactions with target RNAs or proteins.
MEX3C is widely expressed in human tissues and implicated in diverse physiological processes, including immune response, cell cycle progression, and apoptosis. Notably, it participates in the ubiquitin-proteasome system, targeting substrates like A20 (TNFAIP3) for degradation, thereby modulating NF-κB signaling and inflammation. Dysregulation of MEX3C has been linked to multiple pathologies: its overexpression correlates with tumor progression in cancers (e.g., hepatocellular carcinoma and glioblastoma) by promoting oncogenic pathways and evading immune surveillance. Conversely, reduced MEX3C activity is associated with neurodegenerative disorders and viral infections, such as hepatitis C, where it interacts with viral RNA.
Recombinant MEX3C protein, produced via bacterial or mammalian expression systems, is pivotal for studying its molecular mechanisms and interactions. Research focuses on its therapeutic potential, with inhibitors or CRISPR-based approaches being explored to target MEX3C in cancer and inflammatory diseases.
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