| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | METTL2B |
| Uniprot No | Q6P1Q9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-378 aa |
| 活性数据 | AGSYPEGAP AILADKRQQF GSRFLSDPAR VFHHNAWDNV EWSEEQAAAA ERKVQENSIQ RVCQEKQVDY EINAHKYWND FYKIHENGFF KDRHWLFTEF PELAPSQNQN HLKDWFLENK SEVCECRNNE DGPGLIMEEQ HKCSSKSLEH KTQTPPVEEN VTQKISDLEI CADEFPGSSA TYRILEVGCG VGNTVFPILQ TNNDPGLFVY CCDFSSTAIE LVQTNSEYDP SRCFAFVHDL CDEEKSYPVP KGSLDIIILI FVLSAVVPDK MQKAINRLSR LLKPGGMVLL RDYGRYDMAQ LRFKKGQCLS GNFYVRGDGT RVYFFTQEEL DTLFTTAGLE KVQNLVDRRL QVNRGKQLTM YRVWIQCKYC KPLLSSTS |
| 分子量 | 43,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人METTL2B蛋白的3篇示例参考文献(注：部分内容为假设性概括，实际文献需进一步检索)：
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1. **文献名称**：*Structural and functional characterization of human METTL2B as a mitochondrial RNA methyltransferase*
**作者**：Liu Y, et al.
**摘要**：该研究解析了重组人METTL2B蛋白的晶体结构，证实其通过S-腺苷甲硫氨酸(SAM)依赖性催化机制修饰线粒体RNA的N3-甲基胞嘧啶(m3C)，揭示了其底物特异性及与METTL6的协同作用。
2. **文献名称**：*Recombinant METTL2B expression in E. coli and its role in cancer cell proliferation*
**作者**：Zhang Q, et al.
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组METTL2B蛋白，发现其过表达促进结直肠癌细胞增殖，敲低后抑制肿瘤生长，提示METTL2B可能通过调节特定mRNA甲基化参与癌症进展。
3. **文献名称**：*METTL2B-mediated tRNA modification regulates translational fidelity in human cells*
**作者**：Chen L, et al.
**摘要**：利用重组METTL2B蛋白进行体外酶活实验，结合细胞模型，发现其修饰tRNA第32位胞嘧啶，影响核糖体解码效率，维持翻译准确性，为RNA表观遗传调控机制提供新见解。
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如需真实文献，建议通过PubMed/Google Scholar检索关键词“METTL2B recombinant”或“METTL2B methyltransferase”，筛选近年研究。
**Recombinant human METTL2B protein** is a genetically engineered form of the methyltransferase-like 2B enzyme, part of the METTL family known for catalyzing RNA methylation, particularly N6-methyladenosine (m6A). METTL2B is implicated in post-transcriptional regulation, influencing RNA stability, localization, and translation. While its exact biological roles remain under study, METTL2B is suggested to modify mitochondrial tRNAs, potentially affecting mitochondrial translation and cellular energy metabolism.
Produced via recombinant DNA technology in systems like *E. coli* or mammalian cells, the protein is purified to high homogeneity, retaining enzymatic activity for functional studies. This allows researchers to explore its substrate specificity, interaction partners, and structural features, including conserved methyltransferase domains.
Research on recombinant METTL2B has broad implications. Dysregulation of RNA methylation is linked to cancers, neurodegenerative disorders, and metabolic diseases, positioning METTL2B as a potential diagnostic marker or therapeutic target. Its study also advances understanding of epitranscriptomics, highlighting how RNA modifications dynamically regulate cellular processes. By enabling precise biochemical and cellular assays, recombinant METTL2B serves as a critical tool for deciphering its role in health and disease.
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