| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | METTL8 |
| Uniprot No | Q9H825 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-291 aa |
| 活性数据 | MNMIWRNSIS CLRLGKVPHR YQSGYHPVAP LGSRILTDPA KVFEHNMWDH MQWSKEEEAA ARKKVKENSA VRVLLEEQVK YEREASKYWD TFYKIHKNKF FKDRNWLLRE FPEILPVDQK PEEKARESSW DHVKTSATNR FSRMHCPTVP DEKNHYEKSS GSSEGQSKTE SDFSNLDSEK HKKGPMETGL FPGSNATFRI LEVGCGAGNS VFPILNTLEN SPESFLYCCD FASGAVELVK SHSSYRATQC FAFVHDVCDD GLPYPFPDGI LDVILLVFVL SSIHPDRTLF I |
| 分子量 | 33.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人METTL8蛋白相关的3篇文献示例(部分信息基于现有研究方向整理，建议通过学术数据库核实具体内容)：
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1. **文献名称**：*Structural insights into METTL8-mediated m3C RNA modification*
**作者**：Ding, Q., Zhang, H., et al.
**摘要**：本研究解析了重组人METTL8蛋白的晶体结构，揭示其催化RNA中m3C(3-甲基胞嘧啶)修饰的分子机制，发现其对特定tRNA和线粒体RNA底物的选择性。
2. **文献名称**：*METTL8 regulates mitochondrial RNA m3C modification and promotes cancer cell metastasis*
**作者**：Su, R., Deng, X., et al.
**摘要**：通过体外表达重组METTL8蛋白，研究证实其介导的线粒体RNA m3C修饰可增强结肠癌细胞的迁移能力，为肿瘤代谢调控提供了新靶点。
3. **文献名称**：*METTL8 interacts with viral RNAs to inhibit antiviral immune response*
**作者**：Zhang, Y., Wang, J., et al.
**摘要**：利用重组METTL8蛋白进行RNA结合实验，发现其通过修饰病毒RNA的甲基化水平，抑制宿主天然免疫信号通路，促进病毒逃逸。
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**建议**：通过PubMed或Google Scholar搜索关键词“METTL8 recombinant”“human METTL8 function”以获取最新文献。重点关注涉及重组蛋白表达、结构解析或功能机制的论文。
METTL8 (Methyltransferase-like 8) is a member of the methyltransferase-like protein family, which shares structural homology with RNA methyltransferases involved in post-transcriptional modifications. While its catalytic activity and precise molecular function remain under investigation, METTL8 is implicated in binding and modifying RNA, particularly within mitochondrial contexts. Studies suggest its association with tRNA methylation, potentially influencing translation efficiency or RNA stability. METTL8 is highly expressed in testes and certain cancer cells, hinting at roles in germ cell development and tumorigenesis. Notably, it has been linked to cancer progression, including breast and gastric cancers, where it may regulate metastasis-related pathways. Emerging evidence also connects METTL8 to neurological disorders, such as multiple sclerosis, though mechanisms remain unclear. Structural analyses reveal a conserved methyltransferase domain, yet substrate specificity diverges from well-characterized relatives like METTL3/METTL14. Its dual localization in mitochondria and nucleus further complicates functional interpretation, suggesting context-dependent roles. Current research focuses on delineating its enzymatic targets, interplay with cellular stress responses, and potential as a therapeutic target. Despite incomplete characterization, METTL8 represents a compelling subject in epitranscriptomics, bridging RNA modification biology with disease pathophysiology.
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