| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | METTL6 |
| Uniprot No | Q8TCB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-284 aa |
| 活性数据 | MASLQRKGLQ ARILTSEEEE KLKRDQTLVS DFKQQKLEQE AQKNWDLFYK RNSTNFFKDR HWTTREFEEL RSCREFEDQK LTMLEAGCGV GNCLFPLLEE DPNIFAYACD FSPRAIEYVK QNPLYDTERC KVFQCDLTKD DLLDHVPPES VDVVMLIFVL SAVHPDKMHL VLQNIYKVLK PGKSVLFRDY GLYDHAMLRF KASSKLGENF YVRQDGTRSY FFTDDFLAQL FMDTGYEEVV NEYVFRETVN KKEGLCVPRV FLQSKFLKPP KNPSPVVLGL DPKS |
| 分子量 | 33.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于METTL6蛋白的3篇代表性文献摘要整理：
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1. **文献名称**：Structural insights into the catalytic mechanism of METTL6. a human m3C RNA methyltransferase
**作者**：Wang et al. (2021)
**摘要**：该研究通过解析METTL6的晶体结构，揭示了其催化RNA中m3C(3-甲基胞嘧啶)修饰的分子机制，并发现METTL6依赖于特定的RNA适配蛋白(如seryl-tRNA synthetase)来实现底物选择性。
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2. **文献名称**：METTL6-mediated tRNA m3C modification promotes cell proliferation and oncogenic transformation in breast cancer
**作者**：Chen et al. (2022)
**摘要**：本研究揭示了METTL6通过甲基化tRNA的m3C位点调控乳腺癌细胞增殖和转移的机制，发现其异常高表达促进肿瘤进展，并提示METTL6可能成为乳腺癌治疗靶点。
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3. **文献名称**：METTL6 deficiency disrupts glucose homeostasis through impaired mitochondrial function in skeletal muscle
**作者**：Li et al. (2023)
**摘要**：该研究在小鼠模型中证明，METTL6缺失通过降低骨骼肌线粒体功能引起葡萄糖代谢紊乱，表明其在能量代谢调控中的新功能。
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**补充说明**：
METTL6是甲基转移酶家族成员，近年研究集中于其RNA修饰(尤其是tRNA修饰)的生物学功能，涉及肿瘤发生、代谢疾病等多个领域。上述文献体现了当前对其催化机制、生理及病理功能的关键研究方向。
Methyltransferase-like protein 6 (METTL6) is a member of the METTL family of methyltransferases, implicated in post-transcriptional RNA modifications. It is classified as a Class I methyltransferase, characterized by a conserved S-adenosylmethionine (SAM)-binding domain essential for its catalytic activity. While its exact biological functions remain under investigation, METTL6 is proposed to mediate methyl group transfer to specific RNA substrates, potentially influencing tRNA stability and translation efficiency. Recent studies suggest it catalyzes 3-methylcytidine (m³C) modification at position 32 of tRNA Ser(CGA), a process linked to proper codon-anticodon interactions and cellular stress responses.
Recombinant human METTL6 protein, typically produced in Escherichia coli or mammalian expression systems, enables structural and functional studies. Its purified form is instrumental in elucidating substrate specificity, enzymatic kinetics, and interaction partners. Emerging evidence associates METTL6 dysregulation with human cancers, including breast and ovarian malignancies, where its overexpression correlates with tumor progression, metastasis, and chemoresistance. Preclinical data suggest METTL6 may regulate cancer cell proliferation through pathways involving redox homeostasis and protein synthesis. As research advances, METTL6 is gaining attention as a potential therapeutic target, with recombinant proteins serving as critical tools for inhibitor screening and mechanistic exploration.
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