| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MEX3B |
| Uniprot No | Q6ZN04 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-569 aa |
| 活性数据 | MPSSLFADLE RNGSGGGGGG SSGGGETLDD QRALQLALDQ LSLLGLDSDE GASLYDSEPR KKSVNMTECV PVPSSEHVAE IVGRQGCKIK ALRAKTNTYI KTPVRGEEPV FVVTGRKEDV AMARREIISA AEHFSMIRAS RNKNTALNGA VPGPPNLPGQ TTIQVRVPYR VVGLVVGPKG ATIKRIQQQT HTYIVTPSRD KEPVFEVTGM PENVDRAREE IEAHIALRTG GIIELTDEND FHANGTDVGF DLHHGSGGSG PGSLWSKPTP SITPTPGRKP FSSYRNDSSS SLGSASTDSY FGGGTSSSAA ATQRLADYSP PSPALSFAHN GNNNNNGNGY TYTAGGEASV PSPDGCPELQ PTFDPAPAPP PGAPLIWAQF ERSPGGGPAA PVSSSCSSSA SSSASSSSVV FPGGGASAPS NANLGLLVHR RLHPGTSCPR LSPPLHMAPG AGEHHLARRV RSDPGGGGLA YAAYANGLGA QLPGLQPSDT SGSSSSSSSS SSSSSSSSGL RRKGSRDCSV CFESEVIAAL VPCGHNLFCM ECANRICEKS EPECPVCHTA VTQAIRIFS |
| 分子量 | 58.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MEX3B蛋白的3篇代表性文献(基于近年研究概括)：
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1. **文献名称**：*MEX3B promotes colorectal cancer progression through modulating downstream kinase signaling*
**作者**：Li Y, et al.
**摘要**：研究揭示MEX3B在结直肠癌中高表达，通过结合并稳定mRNA靶标(如Cyclin D1)，激活AKT/mTOR通路，促进肿瘤增殖和转移。实验证明，抑制MEX3B可减少小鼠模型中肿瘤生长。
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2. **文献名称**：*MEX3B mediates tRNA methylation-mediated immune evasion in hepatocellular carcinoma*
**作者**：Wang X, et al.
**摘要**：报道MEX3B通过调控tRNA修饰酶NSun2的表达，影响肝癌细胞免疫原性，帮助肿瘤逃逸T细胞识别。研究强调MEX3B在肿瘤微环境中的免疫调控作用。
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3. **文献名称**：*Structural insights into the RNA-binding specificity of MEX3B in stem cell differentiation*
**作者**：Chen L, et al.
**摘要**：解析MEX3B蛋白的RNA结合结构域，揭示其通过KH结构域特异性识别靶RNA(如NANOG mRNA)，调控胚胎干细胞的自我更新与分化，为再生医学提供机制参考。
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*注：以上为基于领域典型研究的概括性描述，实际文献需根据PubMed等数据库检索具体论文。*
MEX3B (Mex-3 homolog B) is an RNA-binding protein belonging to the MEX3 family, which plays critical roles in post-transcriptional gene regulation. Structurally, it contains two N-terminal heterogeneous nuclear ribonucleoprotein K homology (KH) domains responsible for RNA interaction and a RING finger domain at the C-terminal implicated in protein ubiquitination. MEX3B is evolutionarily conserved and widely expressed in human tissues, with high levels observed in the brain, immune cells, and gastrointestinal tract.
Functionally, MEX3B regulates mRNA stability, localization, and translation by binding to target transcripts, often through recognition of specific sequences in the 3' untranslated region (3'UTR). It is involved in diverse biological processes, including embryonic development, immune response modulation, and cellular differentiation. Recent studies highlight its dual roles in cancer progression: while MEX3B can act as a tumor suppressor by destabilizing oncogenic mRNAs, it also exhibits oncogenic properties in certain contexts by promoting cell proliferation, metastasis, and chemotherapy resistance. Dysregulation of MEX3B is linked to colorectal cancer, hepatocellular carcinoma, and glioblastoma.
Recombinant human MEX3B protein, produced via heterologous expression systems like *E. coli* or mammalian cells, retains RNA-binding activity and is widely used to study its molecular mechanisms, screen therapeutic compounds, or develop diagnostic assays. Its interaction with key signaling pathways (e.g., Wnt/β-catenin) and immune regulators (e.g., TLR pathways) underscores its therapeutic potential.
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