| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | METT10D |
| Uniprot No | Q86W50 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-562 aa |
| 活性数据 | MALSKSMHAR NRYKDKPPDF AYLASKYPDF KQHVQINLNG RVSLNFKDPE AVRALTCTLL REDFGLSIDI PLERLIPTVP LRLNYIHWVE DLIGHQDSDK STLRRGIDIG TGASCIYPLL GATLNGWYFL ATEVDDMCFN YAKKNVEQNN LSDLIKVVKV PQKTLLMDAL KEESEIIYDF CMCNPPFFAN QLEAKGVNSR NPRRPPPSSV NTGGITEIMA EGGELEFVKR IIHDSLQLKK RLRWYSCMLG KKCSLAPLKE ELRIQGVPKV TYTEFCQGRT MRWALAWSFY DDVTVPSPPS KRRKLEKPRK PITFVVLASV MKELSLKASP LRSETAEGIV VVTTWIEKIL TDLKVQHKRV PCGKEEVSLF LTAIENSWIH LRRKKRERVR QLREVPRAPE DVIQALEEKK PTPKESGNSQ ELARGPQERT PCGPALREGE AAAVEGPCPS QESLSQEENP EPTEDERSEE KGGVEVLESC QGSSNGAQDQ EASEQFGSPV AERGKRLPGV AGQYLFKCLI NVKKEVDDAL VEMHWVEGQN RDLMNQLCTY IRNQIFRLVA VN |
| 分子量 | 63,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条关于重组人METTL10D蛋白的参考文献示例(信息为虚构概括，仅作格式参考，实际文献需通过学术数据库查询)：
1. **“METTL10D-mediated RNA methylation in human cell proliferation”**
Zhang et al., *Journal of Molecular Biology*, 2021.
摘要：研究重组人METTL10D蛋白的RNA甲基转移酶活性，揭示其通过调控m6A修饰参与细胞周期相关基因表达，影响肿瘤细胞增殖的分子机制。
2. **“Structural insights into METTL10D catalytic domain and substrate specificity”**
Wang & Li, *Protein Science*, 2020.
摘要：解析重组人METTL10D蛋白的晶体结构，阐明其甲基化底物结合的关键氨基酸位点，为靶向甲基化酶的药物设计提供结构基础。
3. **“METTL10D knockout mice exhibit metabolic syndrome phenotypes”**
Chen et al., *Cell Metabolism*, 2019.
摘要：通过构建METTL10D基因敲除小鼠模型，发现该蛋白缺失导致肝脏脂代谢紊乱，提示其在代谢性疾病中的潜在调控作用。
(注：以上为模拟示例，实际文献请查询PubMed、Web of Science等平台。)
Recombinant human METTL10D (methyltransferase-like 10D) is a protein of interest in the field of post-translational modifications, particularly methyltransferase-mediated processes. As a member of the methyltransferase-like (METTL) family, METTL10D is hypothesized to catalyze the transfer of methyl groups to target substrates, though its specific biological roles remain less characterized compared to well-studied relatives like METTL3 or METTL14. Structurally, it contains a conserved S-adenosylmethionine (SAM)-binding domain, a hallmark of methyltransferases, suggesting its dependency on SAM as a methyl donor.
Recent studies indicate METTL10D may methylate proteins or nucleic acids, potentially regulating cellular processes such as RNA metabolism, signal transduction, or chromatin remodeling. Its recombinant form is engineered via heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional and structural studies. Researchers utilize purified recombinant METTL10D to dissect enzymatic activity, substrate specificity, and interaction networks, often employing techniques like mass spectrometry or crystallography.
Preliminary evidence links METTL10D to disease contexts, including cancer and neurological disorders, though mechanistic insights are still emerging. By studying recombinant METTL10D, scientists aim to unravel its physiological relevance, therapeutic potential, or role in epigenetic regulation. Its conserved structure across species also makes it a candidate for evolutionary studies of methyltransferase functions. Further research is needed to define its precise substrates and validate its involvement in specific pathways or pathologies.
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