| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MESP1 |
| Uniprot No | Q9BRJ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-268aa |
| 活性数据 | MAQPLCPPLSESWMLSAAWGPTRRPPPSDKDCGRSLVSSPDSWGSTPADSPVASPARPGTLRDPRAPSVGRRGARSSRLGSGQRQSASEREKLRMRTLARALHELRRFLPPSVAPAGQSLTKIETLRLAIRYIGHLSAVLGLSEESLQRRCRQRGDAGSPRGCPLCPDDCPAQMQTRTQAEGQGQGRGLGLVSAVRAGASWGSPPACPGARAAPEPRDPPALFAEAACPEGQAMEPSPPSPLLPGDVLALLETWMPLSPLEWLPEEPK |
| 分子量 | 29.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MESP1蛋白的3篇示例参考文献(内容基于领域相关研究方向，部分为示例性虚构文献)：
1. **文献名称**: *MESP1 Drives Cardiomyocyte Commitment through Chromatin Remodeling*
**作者**: David et al., *Cell Stem Cell* (2021)
**摘要**: 研究揭示重组人MESP1蛋白通过调控染色质可及性，激活心脏发育相关基因(如NKX2.5、TBX5)，促进胚胎干细胞向心肌细胞定向分化。实验表明，MESP1与染色质重塑复合物(如SWI/SNF)相互作用，确定其在心脏谱系决定中的表观遗传调控机制。
2. **文献名称**: *Recombinant MESP1 Protein Enhances Cardiac Reprogramming in Fibroblasts*
**作者**: Suzuki et al., *Circulation Research* (2019)
**摘要**: 本研究利用重组人MESP1蛋白与特定小分子组合，将成纤维细胞直接重编程为心血管祖细胞。MESP1通过激活Wnt和Notch通路，显著提高转化效率，为心脏再生治疗提供新策略。
3. **文献名称**: *Structural Insights into MESP1 Transcription Factor Function*
**作者**: Liu et al., *Nature Structural & Molecular Biology* (2022)
**摘要**: 通过X射线晶体学解析重组人MESP1蛋白的DNA结合域结构，发现其bHLH结构域与心脏特异性增强子结合的模式。突变分析揭示关键氨基酸残基(如Arg153)对靶基因激活的贡献。
4. **文献名称**: *MESP1-Based Differentiation of iPSCs for Disease Modeling*
**作者**: Chen et al., *Stem Cell Reports* (2020)
**摘要**: 利用重组MESP1蛋白优化诱导多能干细胞(iPSC)分化方案，构建遗传性心肌病模型。研究证实MESP1时序表达可模拟胚胎心脏发育，助力疾病机制研究与药物筛选。
(注：以上文献名为示例性质，实际文献需根据具体研究内容检索。)
MESP1 (Mesoderm Posterior 1) is a key transcription factor critical for early embryonic development, particularly in the specification of mesodermal lineages and cardiovascular progenitor cells. It belongs to the basic helix-loop-helix (bHLH) protein family and plays a pivotal role during gastrulation, orchestrating the differentiation of pluripotent stem cells into mesodermal subtypes. In humans, MESP1 is transiently expressed in the primitive streak and is essential for heart development, as it marks the earliest cardiac precursor cells and regulates genes involved in cardiomyocyte differentiation, migration, and tissue patterning. Dysregulation of MESP1 has been linked to congenital heart defects, underscoring its biological significance.
Recombinant human MESP1 protein is engineered to study its molecular functions and therapeutic potential. Produced via bacterial or mammalian expression systems, it typically includes tags (e.g., His-tag) for purification and tracking. Researchers use this protein in vitro to direct stem cell differentiation toward cardiovascular lineages, model heart development, or screen drugs targeting cardiac regeneration. Structural studies focus on its bHLH domain, which mediates DNA binding and dimerization with partners like E-proteins. Despite challenges in maintaining its stability due to transient natural expression, recombinant MESP1 remains a vital tool for decoding early cardiogenesis mechanisms and advancing regenerative medicine strategies.
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