| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MEST |
| Uniprot No | Q5EB52 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-335 aa |
| 活性数据 | MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKSSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNFFVFSRGLTPVFGPYTRPSESELWDMWAGIRNNDGNLVIDSLLQYINQRKKFRRRWVGALASVTIPIHFIYGPLDPVNPYPEFLELYRKTLPRSTVSILDDHISHYPQLEDPMGFLNAYMGFINSF |
| 分子量 | 65.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MEST蛋白的3篇参考文献示例(文献标题和作者为虚构，摘要内容基于相关领域研究概括)：
1. **"Expression and Functional Analysis of Recombinant Human MEST Protein in Adipocyte Differentiation"**
*作者：Lee S, et al. (2015)*
摘要：本研究通过哺乳动物表达系统成功制备了重组人MEST蛋白，并发现其通过调控PPARγ信号通路显著促进脂肪细胞分化，提示MEST在代谢调节中的潜在作用。
2. **"Structural Characterization of Recombinant MEST and Its Role in Cell Adhesion"**
*作者：Zhang Y, et al. (2018)*
摘要：利用昆虫细胞体系表达并纯化人MEST重组蛋白，通过晶体学分析其三维结构，发现其与整合素相互作用，参与细胞黏附和迁移过程，为癌症转移机制研究提供依据。
3. **"Recombinant MEST Protein Attenuates Pulmonary Fibrosis in a Mouse Model"**
*作者：Tanaka K, et al. (2020)*
摘要：构建并验证重组人MEST蛋白在小鼠肺纤维化模型中的治疗作用，发现其通过抑制TGF-β/Smad通路减轻纤维化表型，提示其作为抗纤维化治疗分子的潜力。
*注：以上文献为示例性内容，如需真实文献建议通过PubMed或Web of Science平台检索关键词 "recombinant human MEST protein"。*
**Background of Recombinant Human MEST Protein**
The mesoderm-specific transcript (MEST), also known as PEG1. is a paternally expressed imprinted gene located on chromosome 7q32. It encodes a protein belonging to the α/β-hydrolase fold family, characterized by a conserved hydrolase domain, though its precise enzymatic activity remains unclear. MEST is implicated in embryonic development, particularly in mesoderm formation, and plays roles in cell adhesion, proliferation, and differentiation. Studies suggest its involvement in lipid metabolism, influencing adipocyte function and insulin sensitivity.
Dysregulation of MEST has been linked to metabolic disorders (e.g., obesity, type 2 diabetes) and cancer progression, where it may modulate tumor growth or metastasis. Recombinant human MEST protein, produced via expression systems like *E. coli* or mammalian cells, enables functional studies to elucidate its mechanisms. It serves as a tool for investigating imprinting effects, developmental pathways, and disease-related pathways. Additionally, its potential therapeutic applications, such as in tissue regeneration or metabolic syndrome management, are under exploration. Despite progress, the full scope of MEST's biological functions and molecular interactions remains an active area of research.
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