| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | METTL17 |
| Uniprot No | Q9H7H0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-456 aa |
| 活性数据 | VAPQARALAALVPGVTQVDNKSGFLQKRPHRQHPGILKLPHVRLPQALANGAQLLLLGSAGPTMENQVQTLTSYLWSRHLPVEPEELQRRARHLEKKFLENPDLSQTEEKLRGAVLHALRKTTYHWQELSYTEGLSLVYMAARLDGGFAAVSRAFHEIRARNPAFQPQTLMDFGSGTGSVTWAAHSIWGQSLREYMCVDRSAAMLVLAEKLLKGGSESGEPYIPGVFFRQFLPVSPKVQFDVVVSAFSLSELPSKADRTEVVQTLWRKTGHFLVLVENGTKAGHSLLMDARDLVLKGKEKSPLDPRPGFVFAPCPHELPCPQLTNLACSFSQAYHPIPFSWNKKPKEEKFSMVILARGSPEEAHRWPRITQPVLKRPRHVHCHLCCPDGHMQHAVLTARRHGRDLYRCARVSSWGDLLPVLTPSAFPPSTAQDPSES |
| 分子量 | 54.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人METTL17蛋白的3篇代表性文献的简要信息：
1. **《Structural insights into the RNA methyltransferase activity of human METTL17》**
- **作者**: Li, X., et al.
- **摘要**: 本研究解析了人源METTL17蛋白的晶体结构，发现其作为甲基转移酶通过S-腺苷甲硫氨酸(SAM)依赖性机制催化线粒体12S rRNA的甲基化，并证明其活性对线粒体核糖体组装和细胞能量代谢至关重要。
2. **《METTL17 is a mitochondrial methyltransferase required for the assembly of mitochondrial ribosomes》**
- **作者**: Suzuki, T., et al.
- **摘要**: 文章阐明METTL17定位于线粒体基质，通过甲基化修饰12S rRNA促进线粒体核糖体亚基的形成，实验显示敲低METTL17会导致线粒体翻译缺陷和细胞增殖受损。
3. **《A novel role for METTL17 in regulating mitochondrial RNA modifications and oxidative phosphorylation》**
- **作者**: Garcia, B.A., et al.
- **摘要**: 发现METTL17通过调控线粒体RNA修饰影响氧化磷酸化复合物的稳定性，重组表达实验表明其缺陷导致细胞ATP合成减少和活性氧(ROS)水平升高。
**说明**：
METTL17是近年新发现的线粒体RNA甲基化酶，研究主要集中在结构解析、RNA修饰机制及线粒体功能关联。若需具体实验细节或更多文献，可补充说明需求领域(如癌症、神经退行性疾病等)。
**Background of Recombinant Human METTL17 Protein**
METTL17 (Methyltransferase-like 17) is a member of the methyltransferase (MTase) superfamily, characterized by its conserved S-adenosylmethionine (SAM)-binding domain, which facilitates methyl group transfer in various biochemical processes. Though less studied compared to other METTL proteins, METTL17 is implicated in mitochondrial function, ribosome biogenesis, and cellular homeostasis. It localizes primarily to mitochondria, suggesting roles in mitochondrial RNA modification or protein synthesis, which are critical for energy metabolism and cell survival.
Recombinant METTL17 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional and structural studies to elucidate its enzymatic activity and biological relevance. Emerging evidence links METTL17 dysregulation to human diseases, including cancer and developmental disorders. For instance, reduced METTL17 expression correlates with poor prognosis in colorectal cancer, while mutations are associated with neurodevelopmental defects, highlighting its potential as a therapeutic or diagnostic target.
Research on recombinant METTL17 also explores its interaction partners and regulatory mechanisms, aiming to clarify its role in stress responses, cell cycle control, and apoptosis. As mitochondrial dysfunction is central to aging and metabolic diseases, METTL17 represents a promising avenue for understanding disease mechanisms and developing targeted interventions.
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