关键信息
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基因名
CES1
- 应用
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别名
CES1；ADGF；CECR1；IDGFL；Adenosine deaminase 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P23141-3
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表达区间
19-562aa
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氨基酸序列
GHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTP PQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCL YLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVT IQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTI FGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQ IAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQP LLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMLMSYPL SEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFL DLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDH GDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWP EYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEVDHHH HHH
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分子量
61 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CES1 (carboxylesterase 1) is an important enzyme that plays a crucial role in the hydrolysis of various esters, including pharmaceutical compounds and environmental pollutants. Its research background stems from the growing need to understand biotransformation processes, particularly in the context of drug metabolism and toxicology. CES1 is primarily expressed in the liver and is involved in the metabolism of prodrugs, which are converted into active drugs through enzymatic activity. This makes CES1 a key player in pharmacokinetics, influencing drug efficacy and safety. Moreover, variations in CES1 expression and activity can be linked to genetic polymorphisms, leading to interindividual differences in drug response. Consequently, studying CES1 not only aids in the development of effective therapeutic agents but also helps in assessing risks associated with exposure to harmful substances. Recent advancements in proteomics and molecular biology have enabled researchers to explore the structural and functional characteristics of CES1, facilitating the identification of potential inhibitors and substrates. As a result, understanding CES1 offers valuable insights into personalized medicine, environmental remediation, and the design of safer pharmaceuticals, highlighting its significance in both clinical and environmental contexts. The ongoing research into CES1 and its role in various biological processes continues to expand our comprehension of enzyme function and its implications for human health and environmental sustainability.
