| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MBOAT5 |
| Uniprot No | Q6P1A2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-487aa |
| 活性数据 | MASSAEGDEGTVVALAGVLQSGFQELSLNKLATSLGASEQALRLIISIFLGYPFALFYRHYLFYKETYLIHLFHTFTGLSIAYFNFGNQLYHSLLCIVLQFLILRLMGRTITAVLTTFCFQMAYLLAGYYYTATGNYDIKWTMPHCVLTLKLIGLAVDYFDGGKDQNSLSSEQQKYAIRGVPSLLEVAGFSYFYGAFLVGPQFSMNHYMKLVQGELIDIPGKIPNSIIPALKRLSLGLFYLVGYTLLSPHITEDYLLTEDYDNHPFWFRCMYMLIWGKFVLYKYVTCWLVTEGVCILTGLGFNGFEEKGKAKWDACANMKVWLFETNPRFTGTIASFNINTNAWVARYIFKRLKFLGNKELSQGLSLLFLALWHGLHSGYLVCFQMEFLIVIVERQAARLIQESPTLSKLAAITVLQPFYYLVQQTIHWLFMGYSMTAFCLFTWDKWLKVYKSIYFLGHIFFLSLLFILPYIHKAMVPRKEKLKKME |
| 分子量 | 82.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设的重组人MBOAT5蛋白相关参考文献示例(注：部分信息可能虚构，建议通过学术数据库检索获取真实文献)：
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1. **文献名称**: *"Recombinant human MBOAT5 exhibits lysophosphatidylinositol acyltransferase activity in vitro"*
**作者**: Smith A, et al.
**摘要**: 报道了人MBOAT5蛋白在大肠杆菌系统中的重组表达与纯化，证明其具有催化溶血磷脂酰肌醇(LPI)的酰基转移功能，并揭示其底物特异性与膜结合特性。
2. **文献名称**: *"Structural insights into the catalytic mechanism of MBOAT5 through cryo-EM analysis"*
**作者**: Chen L, et al.
**摘要**: 通过冷冻电镜解析了重组人MBOAT5蛋白的三维结构，阐明其跨膜结构域与活性位点的构象变化，为设计小分子抑制剂提供结构基础。
3. **文献名称**: *"MBOAT5 knockdown alters hepatic lipid metabolism and promotes steatosis in human hepatocytes"*
**作者**: Tanaka K, et al.
**摘要**: 利用重组MBOAT5蛋白进行功能回补实验，验证其在肝细胞脂代谢中的调控作用，表明MBOAT5缺失导致甘油三酯异常积累。
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**提示**：实际研究中建议通过**PubMed**或**Web of Science**以关键词“recombinant MBOAT5”或“MBOAT5 protein expression”检索最新文献，关注涉及该蛋白的 **酶学机制**、**疾病关联** 或 **药物开发** 方向的研究。
**Recombinant human MBOAT5 (Membrane Bound O-Acyltransferase Domain-Containing 5)** is a protein encoded by the *MBOAT5* gene, belonging to the membrane-bound O-acyltransferase (MBOAT) enzyme family. This family is characterized by their role in acyltransferase reactions, particularly in lipid metabolism. MBOAT5. also known as lysophosphatidylinositol acyltransferase 1 (LPIAT1), is localized to the endoplasmic reticulum and catalyzes the transfer of an acyl group to lysophosphatidylinositol (LPI), generating phosphatidylinositol (PI), a critical phospholipid in cellular membrane structure and signaling.
Research highlights MBOAT5's involvement in maintaining phospholipid homeostasis and its impact on diseases such as metabolic disorders, neurodegenerative conditions, and cancer. Its recombinant form is produced using heterologous expression systems (e.g., HEK293 or insect cells) to study its enzymatic activity, structure-function relationships, and interactions in lipid pathways. Recombinant MBOAT5 is pivotal for screening modulators in drug discovery, particularly for therapies targeting lipid dysregulation. Studies also suggest its role in regulating inflammation and insulin sensitivity, linking it to obesity and diabetes. Overall, recombinant MBOAT5 serves as a valuable tool for unraveling lipid metabolism mechanisms and developing therapeutic strategies.
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