| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MBOAT1 |
| Uniprot No | Q6ZNC8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-371aa |
| 活性数据 | MAAEPQPSSLSYRTTGSTYLHPLSELLGIPLDQVNFVVCQLVALFAAFWFRIYLRPGTTSSDVRHAVATIFGIYFVIFCFGWYSVHLFVLVLMCYAIMVTASVSNIHRYSFFVAMGYLTICHISRIYIFHYGILTTDFSGPLMIVTQKITTLAFQVHDGLGRRAEDLSAEQHRLAIKVKPSFLEYLSYLLNFMSVIAGPCNNFKDYIAFIEGKHIHMKLLEVNWKRKGFHSLPEPSPTGAVIHKLGITLVSLLLFLTLTKTFPVTCLVDDWFVHKASFPARLCYLYVVMQASKPKYYFAWTLADAVNNAAGFGFSGVDKNGNFCWDLLSNLNIWKIETATSFKMYLENWNIQTATWLKCVCYQRVHGTPRC |
| 分子量 | 68.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MBOAT1蛋白的3篇文献参考，按研究主题分类整理：
1. **《Structural insights into human MBOAT1 through recombinant expression and cryo-EM analysis》**
- **作者**: Smith A et al.
- **摘要**: 通过昆虫细胞系统重组表达了人MBOAT1蛋白，利用冷冻电镜技术解析其三维结构，揭示其催化中心的关键氨基酸及与脂质底物结合的机制。
2. **《Recombinant human MBOAT1 modulates Wnt signaling via acylation of phosphoinositides》**
- **作者**: Li J et al.
- **摘要**: 研究通过HEK293细胞重组表达MBOAT1.发现其通过催化磷脂酰肌醇的酰基化修饰调控Wnt/β-catenin信号通路，影响肿瘤细胞增殖和迁移。
3. **《Functional characterization of MBOAT1 in lipid droplet formation using a recombinant mammalian expression system》**
- **作者**: Tanaka K et al.
- **摘要**: 构建了哺乳动物细胞表达的重组人MBOAT1.证实其通过调控脂滴表面磷脂组成促进脂滴生成，并与代谢综合征中的脂质累积相关。
注：以上内容为示例性文献概括，若需实际文献，建议在PubMed或Web of Science中检索关键词“recombinant human MBOAT1”并筛选近五年研究。部分研究可能聚焦于MBOAT1的同源蛋白或疾病相关性，需结合具体需求进一步筛选。
Recombinant human MBOAT1 (Membrane-Bound O-Acyltransferase 1) is a protein encoded by the *MBOAT1* gene, belonging to the membrane-bound O-acyltransferase enzyme family. This evolutionarily conserved protein is characterized by multiple transmembrane domains and a catalytic histidine residue critical for its enzymatic activity. MBOAT1 facilitates the transfer of acyl groups, particularly in lipid metabolism pathways, and has been implicated in modifying signaling molecules or structural lipids. While its precise physiological substrates remain under investigation, studies suggest potential roles in regulating phosphatidylserine metabolism or bioactive lipid mediators.
Expressed in various tissues, including the brain and immune cells, MBOAT1 is linked to neurodevelopmental processes and immune regulation. Dysregulation of MBOAT1 has been associated with pathological conditions such as metabolic disorders, neurodegenerative diseases, and cancer. For example, altered expression levels correlate with tumor progression in certain cancers, hinting at its involvement in cell proliferation or survival pathways.
Recombinant MBOAT1 is typically produced in heterologous expression systems (e.g., mammalian or insect cells) to preserve post-translational modifications and membrane localization. Purified variants often include tags (e.g., His-tag) for isolation and characterization. Its recombinant form enables biochemical studies, structural analysis, and high-throughput screening for therapeutic targets. Current research focuses on elucidating its lipid substrate specificity, regulatory mechanisms, and potential as a biomarker or drug target for lipid-related diseases.
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