| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MC2R |
| Uniprot No | Q96G30 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-297aa |
| 活性数据 | MKHIINSYENINNTARNNSDCPRVVLPEEIFFTISIVGVLENLIVLLAVFKNKNLQAPMYFFICSLAISDMLGSLYKILENILIILRNMGYLKPRGSFETTADDIIDSLFVLSLLGSIFSLSVIAADRYITIFHALRYHSIVTMRRTVVVLTVIWTFCTGTGITMVIFSHHVPTVITFTSLFPLMLVFILCLYVHMFLLARSHTRKISTLPRANMKGAITLTILLGVFIFCWAPFVLHVLLMTFCPSNPYCACYMSLFQVNGMLIMCNAVIDPFIYAFRSPELRDAFKKMIFCSRYW |
| 分子量 | 60.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MC2R蛋白的3篇典型参考文献及其核心内容概要：
1. **文献名称**：*Functional characterization of recombinant human melanocortin 2 receptor (MC2R) in adrenal cells*
**作者**：Clark, A.J.L., et al.
**摘要**：研究利用重组技术在人肾上腺细胞中表达MC2R，证实其介导ACTH激活的cAMP信号通路及皮质醇合成功能，揭示了MC2R与辅因子MRAP的互作机制。
2. **文献名称**：*Expression and signaling specificity of the melanocortin 2 receptor in CHO cells*
**作者**：Li, S., et al.
**摘要**：通过构建重组人MC2R的中国仓鼠卵巢(CHO)细胞模型，阐明MC2R特异性响应ACTH而非其他黑色素皮质激素配体，并鉴定了其跨膜结构域的关键功能区。
3. **文献名称**：*Ligand binding and structural insights into the human MC2R receptor*
**作者**：Behan, D.P., Smith, R.G.
**摘要**：采用重组纯化的MC2R蛋白进行体外结合实验，结合突变分析揭示了ACTH与MC2R结合的分子机制，提出其胞外域对配体识别的必要性。
(注：上述文献为模拟示例，实际引用需核对真实数据库如PubMed。)
Recombinant human melanocortin 2 receptor (MC2R) protein is a genetically engineered form of the MC2R, a G protein-coupled receptor (GPCR) primarily expressed in the adrenal cortex. MC2R plays a critical role in regulating steroidogenesis by binding to adrenocorticotropic hormone (ACTH), which stimulates cortisol production via cAMP-PKA signaling. Dysregulation of MC2R is linked to adrenal disorders like familial glucocorticoid deficiency (FGD) and ACTH resistance. Producing functional MC2R has been challenging due to its complex structure, membrane localization, and dependence on the melanocortin receptor accessory protein (MRAP) for proper trafficking and ACTH responsiveness. Recombinant MC2R is typically expressed in mammalian systems (e.g., CHO or HEK293 cells) using vectors with affinity tags (e.g., FLAG or His-tag) to facilitate purification. Its production enables structural studies (e.g., cryo-EM), ligand-binding assays, and functional characterization of pathogenic mutations. Recent advances in GPCR engineering and stabilizers like nanobodies have improved MC2R solubility and stability in vitro. Research on recombinant MC2R contributes to understanding ACTH signaling mechanisms, adrenal pathologies, and potential therapies targeting cortisol-related diseases, such as Cushing’s syndrome or adrenal insufficiency. Ongoing challenges include elucidating its interaction dynamics with MRAP and developing small-molecule modulators.
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